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Characterization and Complete Genome Analysis of Pseudomonas aeruginosa Bacteriophage vB_PaeP_LP14 Belonging to Genus Litunavirus

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Abstract

A lytic Pseudomonas aeruginosa phage vB_PaeP_LP14 belonging to the family Podoviridae was isolated from infected mink. The microbiological characterization revealed that LP14 was stable at 40 to 50 °C and stable over a broad range of pH (5 to 12). The latent period was 5 min, and the burst size was 785 pfu/infected cell. The whole-genome sequencing showed that LP14 was a dsDNA virus and has a genome of 73,080 bp. The genome contained 93 predicted open reading frames (ORFs), 17 of which have known functions including DNA replication and modification, transcriptional regulation, structural and packaging proteins, and host cell lysis. No tRNA genes were identified. BLASTn analysis revealed that phage LP14 had a high-sequence identity (96%) with P. aeruginosa phage YH6. Both morphological characterization and genome annotation indicate that phage LP14 is a memberof the family Podoviridae genus Litunavirus. The study of phage LP14 will provide basic information for further research on treatment of P. aeruginosa infections.

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Acknowledgements

This study was funded by a grant from the Donkey Industry Innovation Team Program of Modern Agricultural Technology System from Shandong Province, China (SDAIT-27). The authors would like to thank Jia Wang, Peng Song, and Wenshi Zhong for their help during this work.

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Authors and Affiliations

  1. College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong, China

    Xiaojie Shi, Feiyang Zhao, Xiaoyan Yu, Can Zhang, Wenhua Liu & Huiying Ren

  2. Qingdao Phagepharm Bio-tech Co, Ltd, Qingdao, Shandong, China

    Huzhi Sun & Qiang Pan

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Shi, X., Zhao, F., Sun, H. et al. Characterization and Complete Genome Analysis of Pseudomonas aeruginosa Bacteriophage vB_PaeP_LP14 Belonging to Genus Litunavirus. Curr Microbiol 77, 2465–2474 (2020). https://doi.org/10.1007/s00284-020-02011-5

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