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Inactivation of the Ventral Pallidum by GABAA Receptor Agonist Promotes Non-rapid Eye Movement Sleep in Rats

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Abstract

GABA, the most abundant inhibitory neurotransmitter in the brain, is closely linked with sleep and wakefulness. As the largest area input to the ventral pallidum (VP), the nucleus accumbens (NAc) has been confirmed to play a pivotal role in promoting non-rapid eye movement (NREM) sleep through inhibitory projections from NAc adenosine A2A receptor-expressing neurons to VP GABAergic neurons which mostly express GABAA receptors. Although these studies demonstrate the possible role of VP GABAergic neurons in sleep–wake regulation, whether and how its modulate sleep–wake cycle is not completely clear. In our study, pharmacological manipulations were implemented in freely moving rats and then the EEG and the EMG were recorded to monitor the sleep–wake states. We found that microinjection of muscimol, a GABAA receptor agonist, into the VP increased NREM sleep in both light and dark period. Microinjection of bicuculline, a GABAA receptor antagonist, into the VP increased wakefulness in the light period. Collectively, our data identify the important role of VP GABAA receptor-expressing neurons in NREM sleep of rats which may help improve the understanding of the pathological sleep disorders.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (Grant No. 81771819), National key research and development plan of China (Grant No. 2017YFC0108803), Zhongnan Hospital of Wuhan University Science, Technology and Innovation Seed Fund (Project No. cxpy2017048), the Fundamental Research Funds for the Central Universities (Projects No. 2042017kf0284 and 2042018kf1038) and Wuhan Municipal Science and Technology Project (Project No. 2016060605100525).

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  1. Department of Radiology, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, 430071, People’s Republic of China

    Xuefen Zhang, Ying Liu, Bo Yang & Haibo Xu

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Zhang, X., Liu, Y., Yang, B. et al. Inactivation of the Ventral Pallidum by GABAA Receptor Agonist Promotes Non-rapid Eye Movement Sleep in Rats. Neurochem Res 45, 1791–1801 (2020). https://doi.org/10.1007/s11064-020-03040-z

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