Abstract
Objective
Interleukin (IL)-1β in the joint cavity increases to promote healing after anterior cruciate ligament (ACL) injury. Synovial tissue is a major joint microenvironmental regulator after ACL injury. The purpose of this study was to investigate the effects of synovial cells (SCs) on lysyl oxidase (LOX) and matrix metalloproteinase (MMP) production by ACL fibroblasts (ACLfs) in the presence of IL-1β.
Results
This study sheds light on the regulation of LOX and MMP-1, -2, -3 expression by ACLfs co-cultured with SCs and treated with IL-1β. LOX and MMP-1, 2, 3 gene/protein expression in IL-1β/stretch-stimulated ACLfs co-cultured with SCs were measured by real-time quantitative PCR and Western blot. Meanwhile, MMP-2 activity was analyzed by zymogram. The results showed that co-culture with SCs increased LOX and MMP-1, -2, -3 gene and protein expression in the presence of IL-1β. Next, ACLfs were subjected to 12% mechanical stretch to simulate pathological injury. Under these conditions, SCs inhibited IL-1β-mediated upregulation of LOXs. However, IL-1β enhanced the expression of MMP-1, -2, -3 in injured ACLfs.
Conclusions
SCs can either inhibit or increase LOX production in the presence of IL-1β, while promoting the accumulation of MMP in injured ACLfs. These results may provide crucial insights into the mechanisms underlying ACL poor healing capacity after injury.
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Data availability statement
All data used to support the findings of this study are included within the article.
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Acknowledgements
This study was supported by the National Natural Science Foundation of China (11532004, 11832008, 11602181, 11802096), Innovation and Attracting Talents Program for College and University (‘‘111’’ Project) (B06023), China Postdoctoral Science Foundation (Grant Number: 2018M630867), the Visiting Scholar Foundation of Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education (Grant Number: CQKLBST-2018-009) and (Grant Number: CQKLBST-2018-006), Sharing fund of Chongqing university’s large-scale equipment (2009063038).
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Wang, C., Chi, Q., Sha, Y. et al. Mechanical injury and IL-1β regulated LOXs and MMP-1, 2, 3 expression in ACL fibroblasts co-cultured with synoviocytes. Biotechnol Lett 42, 1567–1579 (2020). https://doi.org/10.1007/s10529-020-02870-9
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DOI: https://doi.org/10.1007/s10529-020-02870-9