Skip to main content
SpringerLink
Log in

Parasitic interactions of Orobanche with selected Nicotiana species and identification of effective resistant genotypes

  • Research Article
  • Published:
Genetic Resources and Crop Evolution Aims and scope Submit manuscript

Abstract

Tobacco is an important commercial crop in India and other parts of the world. Recently, Orobanche cernua Loefl. (broomrape), a total root parasite is causing considerable yield losses to tobacco crop cultivated in India. Control strategies focusing largely on agronomic practices are not successful in achieving its control. Unfortunately there is no tobacco variety having good levels of resistance to O. cernua is available for managing its menace. Due to lack of the availability of O. cernua resistance even in germplasm of cultivated tobacco species, a study was conducted with seven selected species of Nicotiana viz., N. repanda Lehm., N. benthamiana-repanda, N. nesophila, N. umbratica-nesophila, N. stocktonii, N. paniculata L. and N. tabacum L. cv Siri for identifying Orobanche resistance for further utilization in transferring resistance factors to cultivated tobacco. Emergence of higher number of Orobanche and reduction in plant height and weight in N. paniculata, N. repanda, and N. tobacum cv. Siri indicated they are susceptible. Higher reduction in plant height and weight with less number of Orobache shoots N. stocktonii proved to be a susceptible species. With relatively low reduction in plant height and weight under O. cernua treatment, N. benthamiana-repanda and N. nesophila can be considered as tolerant species. N. umbratica-nesophila with less number of Orobanche spikes, higher plant height and dry weights under both control and treated conditions can be considered as a resistant species and can be used in breeding resistant cultivars. Resistant/tolerant species either increased or maintained their nutrient uptake abilities under parasite infestation thereby maintained their growth.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

Abbreviations

N:

Nitrogen

P:

Potassium

K:

Phosphorus

PH:

Plant height

C:

Control

T:

Treated

SL:

Strigolactone

References

  • Alonso LC, Fernandez-Escobar J, Lopez G, Rodriguez-Ojeda MI, SallagO F (1996) New highly virulent sunflower broomrape (Orobanche cernua Loefl.) pathotypes in Spain. In: Musselman J, Parker C (eds) Advances in Parasitic Plant Research. Junta de Andalucia, Cordoba, Spain, pp 639–644

    Google Scholar 

  • Baghyalakshmi K, Sarala K, Prabhakararao K, Reddy DD (2019) Orobanche menace in crop plants: host resistance as a potential tool to control. J Pharmacogn Phytochem 8(SP2):93–102

    Google Scholar 

  • Barker ER, Press MC, Scholes JD, Quick WP (1996) Interactions between the parasitic angiosperm Orobanche aegyptiaca and its tomato host: growth and biomass allocation. Phytol New 133:637–642

    Article  Google Scholar 

  • Butler LG (1995) Chemical communication between the parasitic weed Striga and its crop host. A new dimension. American Chemical Society, Washington, pp 158–166

    Google Scholar 

  • CABI (2019) Orobanche cernua (nodding broomrape) In: Invasive species compendium. CAB International, Wallingford. www.cabi.org/isc. Accessed 14 Oct 2019

  • Ernst WHO (1986) Mineral nutrition of Nicotiana tabacum cv, Bursana during infection by Orobanche ramosa. In: ter Borg SJ ed. vol 103. LH/VPO, Wageningen, p 80–85

  • Gevezova M, Dekalska T, Stoyanov K, Heisteva T, Kostov K, Batchvarova R, Denev I (2012) Recent advance in broomrapes research. J Biosci Biotechnol 1(2):91–105

    Google Scholar 

  • Graves JD (1995) Host-Plant responses to parasitism. In: Parasitic plants. M.C. Press, and J.D. Graves, eds., p 206–225

  • Hamamouch N (2004) Engineering resistance to Orobanche aegyptiaca: evidence of arcotoxin IA as an anti-parasite protein and macromolecule movement from host to parasite. Dissertation submitted to the faculty of the the Virginia Polytechnic Institute and State University

  • Hibberd JM, Quick WP, Press MC, Scholes JD (1998) Can source-sink relations explain responses of tobacco to infection by the root holoparasitic angiosperm Orobanche cernua? Plant, Cell Environ 21(3):333–340

    Article  CAS  Google Scholar 

  • Hibberd JM, Quick WP, Press MC, Scholes JD, Jeschke WD (1999) Solute fluxes from tobacco to the parasitic angiosperm Orobanche cernua and the influence of infection on host carbon and nitrogen relations. Plant, Cell Environ 22(8):937–947

    Article  CAS  Google Scholar 

  • Jackson M (1973) Soil chemical analysis. Prentice-Hall of India. Pvt. Ltd., New Delhi, pp 111–204

    Google Scholar 

  • Julio E, Malpica A, Cotucheau J, Bachet S, Volpatti R, Decorps C, Dorlhac De Borne F (2018) RNA-Seq analysis of Orobanche resistance in tobacco: development of molecular markers for breeding recessive resistance from Wika tobacco variety. CORESTA Congress, Kunming, Agronomy/Phytopathology Groups, AP, p 14

    Google Scholar 

  • Knapp S, Mark WC, Clarkson JJ (2004) Nomenclatural changes and a new sectional classification Nicotiana (Solanaceae). Taxon 53(1):73–82

    Article  Google Scholar 

  • Krishnamurthy GVG (1994) Orobanche problem and management in India. In: Pieterse AH, Verkleij JAC, ter Borg SJ (eds.), Biology and management of Orobanche. In: Proceedings of the third international workshop on Orobanche and related Striga research. Royal Tropical Institute, The Netherlands p 591–598

  • Krishnamurthy GVG, Nagarajan K, Chandwani GH (1976) Studies on the control of Orobanche in tobacco. Tobacco Res 2:58–62

    Google Scholar 

  • Krishnamurthy GVG, Nagarajan K, Ramji Lal (1977) Some studies on Orobanche cernua Loefl, a parasitic weed on tobacco in India. In: Program and abstracts of papers, weed science conference and workshop in india, paper no. 178: 113–114

  • Nagarajan K, Reddy CVK (2001) Resistant sources in tobacco germplasm against different diseases. Tobacco Res 27(2):197–199

    Google Scholar 

  • Parker C, Riches CR (1993) Parasitic weeds of the world: Biology and Control. CAB International, Wallingford, p 332

    Google Scholar 

  • Pérez-Vich B, Akhtouch B, Muñoz-Ruz J, Fernandez-Martinez JM, Jan CC (2002) Inheritance of resistance to a highly virulent race F of Orobanche cumana Wallr. in a sunflower line derived from interspecific amphiploids. Helia vol 25(36), p 137–144

  • Porkabiri Z, Sabaghnia N, Ranjbar R, Maleki HH (2019) Morphological traits and resistance to Egyptian broomrape weed (Orobanche aegyptiaca Pers.) in tobacco under greenhouse condition. Aust J Crop Sci 13(02):287–293. https://doi.org/10.21475/ajcs.19.13.02.p1429

    Article  Google Scholar 

  • Reddy BSR, Swamy S, Mani M (2018) Pest and disease surveillance in flue-cured tobacco growing zones of India. CORESTA Congress, Kunming, Agronomy/Phytopathology Groups, APPOST 04

  • Rodríguez-Ojeda MI, Fernández-Escobar JA, Alonso LC (2001) Sunflower inbred line (KI-374), carrying two recessive genes for resistance against a highly virulent Spanish population of Orobanche cernua Loelf./O. cumana Wallr. race F. In: 7th International symposium on parasitic plants, Nantes, France

  • Sarala K (2019) Next gen breeding strategies for enhancing FCV tobacco productivity and quality. In Souvenir, XV national symposium on tobacco-2019, organised by Indian Society of Tobacco Science, Rajahmundry, p 45–57

  • Skoric D, Pacureanu-Joita M, Sava E (2010) Sunflower breeding for resistance to broomrape (Orobanche cumana Wallr.). An I.N.C.D.A. Fundulea 78:63–79

    Google Scholar 

  • Swarnalatha G, Sarala K, Prabhakara Rao K, Sambasiva Rao KRS (2019) ITS sequence characterization of Orobanche infecting tobacco. In: XV national symposium on tobacco–2019 conducted by Indian Society of Tobacco Science, Rajahmundry, Abstract No. P-74, p 56

  • Tang S, Heesacker A, Kishore VK, Fernandez A, Sadik ES, Cole G, Knapp SJ (2003) Genetic mapping of the or 5 gene for resistance to Orobanche. race E in sunflower. Crop Sci 43:1021–1028

    Article  CAS  Google Scholar 

  • Umehara M, Cao M, Kohki A, Akatsu T, Seto Y, Hanada A, Li W, Takeda-KN Morimoto Y (2015) Structural requirements of strigolactones for shoot branching inhibition in rice and Arabidopsis. Plant Cell Physiol 56(6):1059–1072

    Article  CAS  Google Scholar 

  • Upendra M, Ravi Shankar H, Nageswara Rao CR (2004) Software for design of experiments-1. ICAR-Central Tobacco Research Institute, Rajahmundry

    Google Scholar 

Download references

Acknowledgements

Authors profoundly acknowledge the Competent authority at Indian Council of Agricultural Research-Central Tobacco Research Institute, Rajahmundry for providing encouragement in carrying out the research work.

Funding

The research work presented in the manuscript is not funded by any external agency and it is the work carried out using in-house resources only.

Author information

Authors and Affiliations

  1. Acharya Nagarjuna University, Nagarjunanagar, Guntur, Andhra Pradesh, 522510, India

    G. Swarnalatha

  2. ICAR-Central Tobacco Research Institute, Rajahmundry, Andhra Pradesh, 533 105, India

    K. Sarala, K. Prabhakara Rao & J. Poorna Bindu

  3. ICAR-Central Institute of Cotton Research, Coimbatore, Tamil Nadu, 641 003, India

    K. Baghyalakshmi

  4. Mizoram University (a Central University), Aizawl, Mizoram, 796004, India

    K. R. S. Sambasiva Rao

Authors
  1. G. Swarnalatha
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Sarala
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Prabhakara Rao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Baghyalakshmi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K. R. S. Sambasiva Rao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. J. Poorna Bindu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. Sarala.

Ethics declarations

Conflict of interest

We also declare that there is no conflict of interest

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Swarnalatha, G., Sarala, K., Prabhakara Rao, K. et al. Parasitic interactions of Orobanche with selected Nicotiana species and identification of effective resistant genotypes. Genet Resour Crop Evol 67, 1125–1136 (2020). https://doi.org/10.1007/s10722-020-00900-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10722-020-00900-z

Keywords

Search

Navigation