Skip to main content
SpringerLink
Log in

The origin and germplasm collection for cultivated Dendrobium officinale K. Kimura & Migo individuals revealed by EST-SSR markers

  • Research Article
  • Published:
Genetic Resources and Crop Evolution Aims and scope Submit manuscript

Abstract

Dendrobium officinale K. Kimura et Migo is an endangered and valuable medicinal herb. In order to understand the origin of D. officinale cultivars and the differences in germplasm resources, 81 D. officinale individuals including wild-collected, market-collected, and two germplasm resources from Zhejiang and Jiangxi province was scanned with 75 EST-SSRs. The number of alleles detected at each locus ranged from 1 to 14 with an average of 3.703. The mean expected heterozygosity (0.492), and Shannon’s information index (0.915) indicated a high level of hybridization rate among the individuals tested. Principal component analysis and cluster analysis based on UPGMA illustrated that almost all the germplasm resources from Jiangxi were grouped together, then the germplasm resources from Zhejiang, wild-collected and market-collected were divided into three clusters, indicated that the origin of market-collected individuals were derived from Zhejiang and the germplasm resources from Jiangxi were well preserved and waited for development. Eight recovered sequences of fructose-bisphosphate aldolase 1 from wild-collected and two germplasm resources showed that the similarity of these sequences ranged from 71.15 to 93.42%, and base insertion, deletion and base varieties occurred in almost all the 7 recovered sequences. On the basis of EST-SSR analysis and keeping in view the purity and stability, the D. officinale germplasm resources from Jiangxi was recommended for hybridization in order to develop superior cultivars.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Bagchi S, Kumar KJ (2016) Studies on water soluble polysaccharides from Pithecellobium dulce (Roxb.) Benth seeds. Carbohydr Polym 138:215–221

    Article  CAS  PubMed  Google Scholar 

  • Borba EL, Felix JM, Solferini VN, Semir J (2001) Fly-pollinated Pleurothallis (Orchidaceae) species have high genetic variability: evidence from isozyme markers. Am J Bot 88(3):419–428

    Article  CAS  PubMed  Google Scholar 

  • Coelho NHP, Tambarussi EV, Aguiar BI, Roque RH, Portela RM, Braga RC, Kageyama PY (2018) Understanding genetic diversity, spatial genetic structure, and mating system through microsatellite markers for the conservation and sustainable use of Acrocomia aculeata (Jacq.) Lodd. Ex Mart. Conserv Genet 19(4):879–891

    Article  CAS  Google Scholar 

  • Feng S, Jiao K, Guo H, Jiang M, Hao J, Wang H, Shen C (2017) Succinyl-proteome profiling of Dendrobium officinale, an important traditional Chinese orchid herb, revealed involvement of succinylation in the glycolysis pathway. BMC Genom 18(1):598

    Article  CAS  Google Scholar 

  • Fu LK, Jin JM (1992) China plant red data book—rare and endangered plants, vol 1. Science Press, Beijing

    Google Scholar 

  • Fu T, Hu ZY, He YQ (2017) Development of EST-SSR molecular markers in Dendrobium officinale. J Nucl Agric Sci 31(4):0663–0670

    Google Scholar 

  • Gómez OJ, Blair MW, Frankow-Lindberg BE, Gullberg U (2005) Comparative study of common bean (Phaseolus vulgaris L) landraces conserved ex situ in genebanks and in situ by farmers. Genet Resour Crop Evol 52(4):371–380

    Article  CAS  Google Scholar 

  • Gu S, Ding XY, Wang Y, Zhou Q, Ding G, Li XX, Qian L (2007) Isolation and characterization of microsatellite markers in Dendrobium officinale, an endangered herb endemic to China. Mol Ecol Notes 7(6):1166–1168

    Article  CAS  Google Scholar 

  • Hadj-Arab H, Chèvre AM, Gaude T, Chable V (2010) Variability of the self-incompatibility reaction in Brassica oleracea L. with S 15 haplotype. Sex Plant Reprod 23(2):141–151

    Article  PubMed  Google Scholar 

  • Hou B, Tian M, Luo J, Ji Y, Xue Q, Ding X (2012) Genetic diversity assessment and ex situ conservation strategy of the endangered Dendrobium officinale (Orchidaceae) using new trinucleotide microsatellite markers. Plant Syst Evol 298(8):1483–1491

    Article  CAS  Google Scholar 

  • Huang K, Li Y, Tao S, Wei G, Huang Y, Chen D, Wu C (2016) Purification, characterization and biological activity of polysaccharides from Dendrobium officinale. Molecules 21(6):701

    Article  PubMed Central  CAS  Google Scholar 

  • Huda MK, Wilcock CC (2008) Impact of floral traits on the reproductive success of epiphytic and terrestrial tropical orchids. Oecologia 154(4):731–741

    Article  PubMed  Google Scholar 

  • Khodaeiaminjan M, Kafkas S, Motalebipour EZ, Coban N (2018) In silico polymorphic novel SSR marker development and the first SSR-based genetic linkage map in pistachio. Tree Genet Genom 14(4):45

    Article  Google Scholar 

  • Li X, Ding X, Chu B, Zhou Q, Ding G, Gu S (2008) Genetic diversity analysis and conservation of the endangered Chinese endemic herb Dendrobium officinale Kimura et Migo (Orchidaceae) based on AFLP. Genetica 133(2):159–166

    Article  CAS  PubMed  Google Scholar 

  • Li Q, Mo J, Wu W, Yang J, Li J, Lai T, Liao J (2019) Genetic diversity, population structure and identification of Dendrobium cultivars with high polysaccharide contents using SCoT, SCAR and nested PCR markers. Genet Resour Crop Evol 66(1):71–88

    Article  CAS  Google Scholar 

  • Liu J, He T, Chun Z (2009) DNA molecular identification of Herba Dendrobium and its adulterant species based on ITS sequence analysis. Zhongguo Zhong yao za zhi 34(22):2853–2856

    CAS  PubMed  Google Scholar 

  • Lu JJ, Suo NN, Hu X, Wang S, Liu JJ, Wang HZ (2012) Development and characterization of 110 novel EST-SSR markers for Dendrobium officinale (Orchidaceae). Am J Bot 99(10):415–420

    Article  Google Scholar 

  • Negri V, Tiranti B (2010) Effectiveness of in situ and ex situ conservation of crop diversity. What a Phaseolus vulgaris L. landrace case study can tell us. Genetica 138(9–10):985–998

    Article  PubMed  Google Scholar 

  • Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89(3):583–590

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ng TB, Liu J, Wong JH, Ye X, Sze SCW, Tong Y, Zhang KY (2012) Review of research on Dendrobium, a prized folk medicine. Appl Microbiol Biotechnol 93(5):1795–1803

    Article  CAS  PubMed  Google Scholar 

  • Nichol ST, Rowe JE, Winton JR (1995) Molecular epizootiology and evolution of the glycoprotein and non-virion protein genes of infectious hematopoietic necrosis virus, a fish rhabdovirus. Virus Res 38(2–3):159–173

    Article  CAS  PubMed  Google Scholar 

  • Pan LH, Li XF, Wang MN, Zha XQ, Yang XF, Liu ZJ, Luo JP (2014) Comparison of hypoglycemic and antioxidative effects of polysaccharides from four different Dendrobium species. Int J Biol Macromol 64:420–427

    Article  CAS  PubMed  Google Scholar 

  • Parthiban S, Govindaraj P, Senthilkumar S (2018) Comparison of relative efficiency of genomic SSR and EST-SSR markers in estimating genetic diversity in sugarcane. 3 Biotech 8(3):144

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Peakall ROD, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6(1):288–295

    Article  Google Scholar 

  • Qian ZZ, Yang D, Yan ZL, Yong PENG (2010) Pharmacopoeia of the People’s Republic of China (2010 edition): a milestone in development of China’s healthcare. Chin Herb Med 2(2):157–160

    Google Scholar 

  • Rucińska A, Puchalski J (2011) Comparative molecular studies on the genetic diversity of an ex situ garden collection and its source population of the critically endangered polish endemic plant Cochlearia polonica E. Fröhlich Biodivers Conserv 20(2):401–413

    Article  Google Scholar 

  • Sahoo A, Jena S, Kar B, Sahoo S, Ray A, Singh S, Nayak S (2017) EST-SSR marker revealed effective over biochemical and morphological scepticism towards identification of specific turmeric (Curcuma longa L.) cultivars. 3 Biotech 7(1):84

    Article  PubMed  PubMed Central  Google Scholar 

  • Shapturenko MN, Pechkovskaya TV, Vakula SI, Jakimovich AV, Zabara YM, Khotyleva LV (2017) Informative EST-SSR markers for genotyping and intraspecific differentiation of Brassica oleracea var. capitata L. Russ J Genet Appl Res 7(1):14–20

    Article  CAS  Google Scholar 

  • Shiau YJ, Nalawade SM, Hsia CN, Mulabagal V, Tsay HS (2005) In vitro propagation of the Chinese medicinal plant, Dendrobium candidum Wall. Ex Lindl., from axenic nodal segments. In Vitro Cell Dev Biol Plant 41(5):666–670

    Article  Google Scholar 

  • Slater AT, Calder DM (1988) The pollination biology of Dendrobium speciosum Smith: a case of false advertising? Aust J Bot 36(2):145–158

    Article  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, Lee TVD, Hornes M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 23(21):4407–4414

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wan RL, Sun J, He T, Hu YD, Zhao Y, Wu Y, Chun Z (2017) Cloning cDNA and functional characterization of UDP-glucose pyrophosphorylase in Dendrobium officinale. Biol Plant 61(1):147–154

    Article  CAS  Google Scholar 

  • Wang XJ, Yu L, Yin SH (2009) Pollintion modes effects on fruit set and seed viability of Dendrobium fimbriatum (Orchidaceae). J Yunnan Univ (Nat Sci Ed) S1

  • Wu Y, Si J (2010) Present status and sustainable development of Dendrobium of ficinale industry. China J Chin Mater Med 15

  • Xie Y, Sung W (2007) Conservation status of Chinese species: (1) overview. Integr Zool 2(1):26–35

    Article  PubMed  Google Scholar 

  • Xie M, Hou B, Han L, Ma Y, Ding X (2010) Development of microsatellites of Dendrobium officinale and its application in purity identification of germplasm. Acta Pharm Sin 45(5):667–672

    CAS  Google Scholar 

  • Xing S, Zhang X, Ke H, Lin J, Huang Y, Wei G (2018) Physicochemical properties of polysaccharides from Dendrobium officinale by fractional precipitation and their preliminary antioxidant and anti-HepG2 cells activities in vitro. Chem Central J 12(1):100

    Article  CAS  Google Scholar 

  • Yang S, Gong Q, Wu Q, Li F, Lu Y, Shi J (2014) Alkaloids enriched extract from Dendrobium nobile Lindl. attenuates tau protein hyperphosphorylation and apoptosis induced by lipopolysaccharide in rat brain. Phytomedicine 21(5):712–716

    Article  CAS  PubMed  Google Scholar 

  • Yang J, Wu H, Lv CG, Wang S, Zhang XB, Yang G, Huang LQ (2018) Geographical origin discriminant of Dendrobium officinale based on stable isotope ratios. China J Chin Mater Med 43(6):1118–1123

    Google Scholar 

  • Zha XQ, Luo JP, Wei P (2009) Identification and classification of Dendrobium candidum species by fingerprint technology with capillary electrophoresis. South Afr J Bot 75(2):276–282

    Article  CAS  Google Scholar 

  • Zhang YJ, Song J, Cheng XJ (2018) The complete chloroplast genome sequence of an endangered traditional Chinese medicine plant Dendrobium candidum (Orchidaceae). Conserv Genet Resour 10(1):9–11

    Article  Google Scholar 

  • Zheng SG, Hu YD, Zhao RX, Yan S, Zhang XQ, Zhao TM, Chun Z (2018) Genome-wide researches and applications on Dendrobium. Planta 248(4):769–784

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The work was supported by Major projects of key research programs in Jiangxi province (Grant No. 20171ACF60017; 20192ACB60013) and Education Department Project in Jiangxi Province (Grant No. GJJ170196).

Author information

Authors and Affiliations

  1. Laboratory of Plant Genetic Improvement and Biotechnology, College of Life Sciences, Jiangxi Normal University, No. 99, Zi Yang Road, Nanchang, 330000, Jiangxi, China

    Jiankun Xie, Junhua Zuo, Yunhong Huang, Changsheng Li & Yaling Chen

Authors
  1. Jiankun Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Junhua Zuo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yunhong Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Changsheng Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yaling Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yaling Chen.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 39 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Xie, J., Zuo, J., Huang, Y. et al. The origin and germplasm collection for cultivated Dendrobium officinale K. Kimura & Migo individuals revealed by EST-SSR markers. Genet Resour Crop Evol 67, 1209–1219 (2020). https://doi.org/10.1007/s10722-020-00906-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10722-020-00906-7

Keywords

Search

Navigation