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Regioselective Biotransformation of Phloretin Using Streptomyces avermitilis MA4680

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  • Applied Microbiology
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Abstract

Streptomyces avermitilis MA4680 was used for the biotransformation of phloretin, one of the dihydrochalcone found in apple bark. After LC analysis, two main biotransformed products were identified and they were further analyzed using GC/MS. After BSTFA derivatization of biotransformed product, they were interpreted as regioselectively hydroxylated products of phloretin in Bring. Maximum conversion of phloretin was 6.7% with 1 h of reaction, and the phloretin and reaction products were completely metabolized after 3 h of reaction due to cellular metabolism. Addition of 0.5 mM quinidine completely blocked hydroxylation of phloretin, which means the hydroxylation proceed by P450 monoxygenase dependent metabolism. Addition of Brij 35 detergent resulted in 150% increase of hydroxylated product due to facilitating transport of phloretin and its reaction product across cellular membrane.

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Acknowledgments

This research was supported by a grant (NRF-2017R1D1A1B0503572614) of the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Republic of Korea.

The authors declare no conflict of interest.

Neither ethical approval nor informed consent was required for this study.

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Authors and Affiliations

  1. Department of Chemical Engineering, Dong-A University, Busan, 49315, Korea

    Wooil Kim, Jong-ki Lee & Junehyung Kim

  2. Department of Environmental Engineering, Ajou University, Suwon, 16499, Korea

    Kwon-Young Choi

  3. School of Chemical and Biological Engineering, Seoul National University, Seoul, 08826, Korea

    Byung-Gee Kim

  4. Center for Sliver-Targeted Biomaterials, Graduate School, Dong-A University, Brain Busan 21 Plus Program, Busan, 49315, Korea

    Junehyung Kim

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  1. Wooil Kim
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Correspondence to Junehyung Kim.

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Kim, W., Lee, Jk., Choi, KY. et al. Regioselective Biotransformation of Phloretin Using Streptomyces avermitilis MA4680. Biotechnol Bioproc E 25, 272–278 (2020). https://doi.org/10.1007/s12257-019-0441-7

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