Abstract
To obtain transgenic rose (Rosa hybrida) plants resistant to drought stress, we transferred the superoxide dismutase 2 (SOD2) (from Escherichia coli) gene, which was harbored by the pPZP200 vector, into embryos and embryogenic calluses derived from a rose breeding line, KR056002, using an Agrobacterium tumefaciens-mediated transformation technique. We obtained seven regenerated plants. The presence of the transgene in the seven lines was validated by PCR analysis and it was determined by Southern blot analysis that two to five copies of the transgene were transferred to seven SOD2-transgenic plants. When the SOD2-transgenic lines were rooted, acclimatized, and placed in a chamber at 25 °C, without water for 7 days, the relative ion leakage of SOD2-transgenic plants was lowered by 5–74% than that of non-transgenic (NT) plants, and the relative water content of the SOD2-transgenic plant leaves was higher (0.1–11%) than that of NT plants. In addition, real-time quantitative PCR analysis confirmed that the transgene expression in SOD2-transgenic lines (five of seven lines) after drought treatment was significantly higher than that before drought treatment. Moreover, when the plants were re-watered after drought treatment, all NT plants died, whereas all SOD2-transgenic plants recovered. Collectively, these results suggest that the over-expression of SOD2 enhances drought tolerance in rose plants.
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This study was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ00816704), Rural Development Administration, South Korea.
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SY Lee obtained SOD2-transgenic rose plants, KS Cheon performed PCR analysis, SY Kim performed RT-qPCR, JH Kim performed Southern blot analysis, and others (OH Kwon, HJ Lee, and WH Kim) performed the analysis of RIL and measurement of RWC.
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Communicated by Tae-Ho Han, Ph.D.
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Lee, S.Y., Cheon, KS., Kim, S.Y. et al. Expression of SOD2 enhances tolerance to drought stress in roses. Hortic. Environ. Biotechnol. 61, 569–576 (2020). https://doi.org/10.1007/s13580-020-00239-5
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DOI: https://doi.org/10.1007/s13580-020-00239-5