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Physiological Change and Transcriptome Analysis of Chinese Wild Vitis amurensis and Vitis vinifera in Response to Cold Stress

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Abstract

The Chinese wild Vitis amurensis Rupr. is a crucial resource for cold-resistant germplasm, but the molecular mechanism of cold resistance in V. amurensis has not been clarified. We conducted a physiological and transcriptome analysis of potted plants of V. amurensis accession “Shuangyou” (cold-resistant) and Vitis vinifera cultivar “Red Globe” (cold-sensitive) subjected to 0 °C for 3, 12, 48, and 72 h. The “Shuangyou” exhibited lower electrolyte leakage (EL) and malondialdehyde (MDA) compared with “Red Globe.” The proline contents in “Shuangyou” was higher than “Red Globe” at 0 and 48 h while lower than “Red Globe” at other time points. On the whole, catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) activity in “Shuangyou” was higher than “Red Globe,” and ascorbate peroxidase (APX) activity in “Shuangyou” was lower than “Red Globe.” Transcriptome analysis showed that 240, 310, 1072, and 1107 differentially expressed genes (DEGs) were detected in “Red Globe” at 3, 12, 48, and 72 h, respectively, and 32, 1161, 1894, and 3290 DEGs were found in “Shuangyou” at the same time points, respectively. Functional categories of DEGs included metabolic processes and signal transduction involved with cold resistance in grapevine. The high expression level of encoding peroxiredoxin genes in V. amurensis revealed a strong ability to scavenge reactive oxygen species (ROS). The expression levels of 5 DEGs in “Shuangyou” were more than 20 times higher than those of “Red Globe” at all time points, indicating that some cold-related special pathways maybe involved in V. amurensis. These related genes, as candidate transcripts, may contribute to excellent cold-hardiness breeding in grape.

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Funding

This work received financial support from Shaanxi Province Key Project-Agriculture of the Peoples Republic of China (Grant no. 2017ZDXM-NY-026) and also from The National Science-Technology Support Plan Projects from the Ministry of Science and Technology of the Peoples Republic of China (Grant no. 2013BAD02B04-06).

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  1. Bao Gu and Bo Zhang contributed equally to this work.

Authors and Affiliations

  1. College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China

    Bao Gu, Bo Zhang, Lan Ding, Peiying Li, Li Shen & Jianxia Zhang

  2. Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, Shaanxi, China

    Bao Gu, Bo Zhang, Lan Ding, Peiying Li, Li Shen & Jianxia Zhang

  3. State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi, China

    Bao Gu, Bo Zhang, Lan Ding, Peiying Li, Li Shen & Jianxia Zhang

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  1. Bao Gu
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  2. Bo Zhang
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  3. Lan Ding
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  4. Peiying Li
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Contributions

Bao Gu performed qRT-PCR, data analysis and revision of the manuscript. Bo Zhang carried out grapevine materials preparation, the data analysis, and manuscript writing. Lan Ding, Peiying Li, and Li Shen measured the physiochemical indexes of grape leaves under cold stress and none-stress treatments. Jianxia Zhang designed the experiment and revised the manuscript.

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Correspondence to Jianxia Zhang.

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Highlights

The most cold-resistant Vitis amurensis was used for searching the key cold-resistant genes. Large numbers of DEGs related to cold response were identified, and most of them were related to metabolic processes and signal transduction. Some related peroxiredoxin and transcription factors were potential candidate genes to enhance the cold resistance of grapevine.

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Fig. S1

Quantitative RT-PCR validations. Twelve genes were selected for the quantitative RT-PCR analysis, including zinc finger (AN1-like) family protein(A), cationic amino acid transporter(B), polyamine oxidase(C), E3 ubiquitin-protein ligase(D), allene oxide synthase(E), WRKY transcription factor 26(F), 3-ketoacyl-CoA synthase(G), omega-3 fatty acid desaturase(H), early light-induced protein(I), AAA-ATPase(J), DELLA protein(K). (PNG 4073 kb)

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Gu, B., Zhang, B., Ding, L. et al. Physiological Change and Transcriptome Analysis of Chinese Wild Vitis amurensis and Vitis vinifera in Response to Cold Stress. Plant Mol Biol Rep 38, 478–490 (2020). https://doi.org/10.1007/s11105-020-01210-5

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