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Nitrogen source utilization in co-existing canopy tree and dwarf bamboo in a northern hardwood forest in Japan

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Understory dwarf bamboo mitigated soil N competition with co-existing canopy oak trees by foraging in deeper soils and increasing dependence on N forms that differ from those used by canopy trees.

Abstract

Nitrogen (N) competition among co-existing plant species utilizing different mycorrhiza types was explored through the investigation of N sources of oak trees and dwarf bamboo. Vertical distribution of fine roots, soil N pools, δ15N of leaves, and possible soil N sources and nitrate reductase activity (NRA) were all quantified. The fine roots of canopy trees were more concentrated in the surface soils than roots of the understory dwarf bamboo. Soil NH4+ and extractable organic N (EON) content (based on unit weight) decreased from the organic horizon (O horizon) to the deep soils, the size of the NH4+ pool per unit volume increased with soil depth, and the EON was approximately constant. Soil NO3− was not detected at any soil depth or was not significant in value, while NO3− captured by ion-exchange resin (IER) buried at a 10 cm soil depth and net nitrification were observed via laboratory incubation at all soil depths. The δ15N of the NH4+ and EON pools increased with soil depth and the δ15N of NO3− of IER was lower than that of other N forms, except for the δ15N of NH4+ in the O horizon. Furthermore, root NRA tended to be lower in canopy trees than in the understory, implying lower dependency on NO3− by canopy trees. The pattern of root distribution and mycorrhizal fungi association of the understory vegetation (as well as the high root NRA) suggested that dependence on N in deeper soils was higher in understory plants than in canopy trees. These findings indicate that understory vegetation mitigates soil N competition against co-existing canopy trees via the use of alternative N sources.

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Acknowledgements

We thank members of the for Hokkaido Forest station, Field Science Education and Research Center, Kyoto University for cooperation and logistics at the field site and laboratory. We also thank to Dr. Keitaro Fukushima and Ms. Chikae Tatsumi and the members of Center for Ecological Research, Kyoto University and FFPRI for isotope measurements. This work was supported by JSPS-KAKENHI (NO. 26292085, 16H04937, and 18H02241). This work was also supported by Joint Usage/Research Grant of Center for Ecological Research, Kyoto University. We would like to thank Editage (http://www.editage.jp) for English language editing.

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Authors and Affiliations

  1. Field Science Education and Research Center, Kyoto University, Kyoto, 606-8502, Japan

    Ryunosuke Tateno

  2. Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan

    Masataka Nakayama

  3. Center for Ecological Research, Kyoto University, Otsu, 520-2113, Japan

    Midori Yano & Keisuke Koba

  4. Nakagawa Experimental Forest, Field Science Center for Northern Biosphere, Hokkaido University, 486 Otoineppu, Otoineppu, Hokkaido, 098-2501, Japan

    Karibu Fukuzawa

  5. Shikoku Research Center, Forestry and Forest Products Research Institute, 2-915 Asakuranishi, Kochi, 780-8077, Japan

    Yoshiyuki Inagaki

  6. Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan

    Shin Ugawa

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  1. Ryunosuke Tateno
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Correspondence to Ryunosuke Tateno.

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Tateno, R., Nakayama, M., Yano, M. et al. Nitrogen source utilization in co-existing canopy tree and dwarf bamboo in a northern hardwood forest in Japan. Trees 34, 1047–1057 (2020). https://doi.org/10.1007/s00468-020-01980-1

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