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Identification of miRNAs linked to peanut nodule functional processes

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Abstract

microRNAs (miRNAs) are non-coding small RNAs that regulate gene expression at post-transcriptional level. Thousands of miRNAs have been identified in legumes, but studies about miRNAs linked to peanut nodule functionality are scarce. In this work we analyzed transcriptional changes in peanut nodules to identify miRNAs involved in functional processes of these organs. We found 32 miRNAs precursors differentially expressed in nodules compared with roots, and predicted the potential targets of their corresponding mature miRNAs. Among them, 20 belong to 14 conserved miRNAs families and 12 are Arachis hypogaea-specific miRNAs. Expression levels of 3 miRNAs (ahy-miR399, ahy-miR159 and ahy-miR3508) were confirmed experimentally by qPCR. We also demonstrated that the expression of these miRNAs was not affected by inoculation of a biocontrol bacterium or a fungal pathogen. The catalogue of differentially expressed miRNA precursors and the expression of the corresponding mature miRNA potential targets in the nodules of A. hypogaea obtained in this work is a database of strong candidates, including A. hypogaea-specific miRNAs, for the regulation of the nodule functionality. The analysis of their role in this process will certainly lead to the characterization of essential regulators in these particular aeschynomenoid nodules.

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Acknowledgements

MSF and JR hold a scholarship granted by CONICET and ANPCyT, respectively. FI and AF are members of the Research Career from CONICET. DF and GH are members of the National System of Researchers from CONACYT.

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Authors and Affiliations

  1. Instituto de Investigaciones Agrobiotecnológicas (INIAB)-Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, 5800, Córdoba, Argentina

    María Soledad Figueredo, Johan Rodríguez, Fernando Ibáñez & Adriana Fabra

  2. Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, 62210, Cuernavaca, Morelos, Mexico

    Damien Formey & Georgina Hernández

Authors
  1. María Soledad Figueredo
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  2. Damien Formey
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  3. Johan Rodríguez
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  4. Fernando Ibáñez
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  5. Georgina Hernández
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  6. Adriana Fabra
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Correspondence to Adriana Fabra.

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Communicated by BJ RAO.

Corresponding editor: BJ RAO

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Figueredo, M.S., Formey, D., Rodríguez, J. et al. Identification of miRNAs linked to peanut nodule functional processes. J Biosci 45, 62 (2020). https://doi.org/10.1007/s12038-020-00034-5

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  • DOI: https://doi.org/10.1007/s12038-020-00034-5

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