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α-Helical protein absorption at post-traumatic epileptic foci monitored by Fourier transform infrared mapping

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Abstract

Tissue analysis by Fourier transform infrared (FTIR) imaging can determine the biodistribution of molecules, without pre-analytical modification. We aimed to study the infrared spectroscopic changes of α-helical proteins at post-traumatic epileptic (PTE) foci by FTIR. FITR mapping was applied to detect α-helical proteins in rat brain tissue samples with post-traumatic epilepsy. Histological examination of brain sections showed that the rat model of PTE was successfully established. At the PTE foci, high α-helical absorption regions were evident, where the color difference and absorption were significantly different from those in the low-absorption regions. This provided a distinctive and characteristic pattern at the site of lesions. The use of FTIR imaging means that it is possible to measure the molecular structural changes resulting from PTE pathologies in tissues, providing a novel adjunct to conventional pathological diagnostic techniques.

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Funding

This study was supported by the Young Innovative Research Team in China University of Political Science and Law (18CXTD09); Program for Young Innovative Research Team in China University of Political Science and Law (19CXTD04), ‘Double First-Class’ University Project and Innovative Talents Training Project (2011-051040); National Natural Science Foundation of China (81871523),Natural Science Foundation of Beijing (7182022), the Open Project of Shanxi Key Laboratory of Forensic Medicine and Hubei Key Laboratory of the Forensic Science, Hubei University of Police.

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  1. These authors contributed equally to this work

Authors and Affiliations

  1. Center of Cooperative Innovation for Judicial Civilization, Beijing, 100088, China

    Siyang Xiang, Dong Zhao, Hongxia Hao, Xu Wang & Tiantong Yang

  2. China University of Political Science and Law, Key Laboratory of Evidence Science, Ministry of Education, Beijing, 100088, China

    Siyang Xiang & Dong Zhao

  3. Office of the Chief Medical Examiner, State of Maryland, Baltimore, USA

    Ling Li

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  1. Siyang Xiang
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  2. Dong Zhao
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  3. Hongxia Hao
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  4. Xu Wang
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  5. Ling Li
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  6. Tiantong Yang
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Correspondence to Tiantong Yang.

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Corresponding editor: BJ RAO

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Xiang, S., Zhao, D., Hao, H. et al. α-Helical protein absorption at post-traumatic epileptic foci monitored by Fourier transform infrared mapping. J Biosci 45, 55 (2020). https://doi.org/10.1007/s12038-020-00028-3

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