Abstract
Tobacco (Nicotiana tabacum L.) is an important agronomic crop and model system for studies of plant-pathogen interactions. Black shank, caused by Phytophthora nicotianae, is an important disease affecting tobacco production worldwide. In this study, a mapping population of 177 F7:8-9 recombinant inbred lines was generated from a cross between the highly resistant cultivar ‘Yunyan 85’ and a susceptible line ‘Dabaijin 599’. A high-density genetic linkage map containing 7734 single-nucleotide polymorphic markers based on restriction site-associated DNA tag sequencing technology was used to finely map quantitative trait loci (QTL) for resistance to P. nicotianae. A total of 10 QTLs were detected as being associated with resistance to P. nicotianae across multiple environments, and two major QTL qBS7 and qBS14 were repeatedly identified under all five environments. They explained 16.48-62.20% and 3.94-11.29% of the phenotypic variance with high LOD score, respectively. One hundred thirty-eight candidate genes were identified for two major QTLs qBS7 and qBS14, and annotation analysis showing that several predicted genes encoded proteins associated with plant defense response to pathogens. This high-density single-nucleotide polymorphic genetic linkage map of flue-cured tobacco based on restriction site-associated DNA sequencing was useful in the QTL finely mapping of resistance to P. nicotianae. This study increases our understanding of the genetics of resistance to P. nicotianae and aids in marker-assisted selection.
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Abbreviations
- QTL:
-
Quantitative trait loci
- RIL:
-
Recombinant inbred line
- SNP:
-
Single nucleotide polymorphism
- NBS-LRR:
-
Nucleotide-binding site-leucine-rich repeat
- LOD:
-
Log-likelihood
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Funding
This work was supported by the Fundamental Research Funds for Central Non-profit Scientific Institution (1610232017009 and 1610232017012) and the Science Foundation for Young Scholars of Tobacco Research Institute of Chinese Academy of Agricultural Sciences (2015B01).
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YS, YZ, and XX collected the plant materials used in this study. DG and MC carried out the determination of resistance to black shank. DG, MC, and XZ analyzed data and prepared the manuscript. DG and XX planned and supervised this work and edited the manuscript. All authors read and approved the final manuscript.
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Table S1.
The RIL population genotype of mapped markers (XLSX 4961 kb)
Table S2.
The markers and their genetic distance in the genetic map. (XLSX 162 kb)
Table S3.
The functional annotation of the candidate genes of the major QTLs. (XLSX 15 kb)
Figure S1.
Haplotype maps of the RIL population. Green represents ‘Yunyan 85’, blue represents ‘Dabaijin 599’, white means the parent could not be estimated, gray represents deletions, and red indicates heterozygosity. (PDF 5026 kb)
Figure S2.
Heat maps of pair-wise recombination of the tobacco. Each cell represents the recombination rate of two markers. Yellow indicates a lower recombination rate and purple a higher one. (PDF 12403 kb)
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Gong, D., Chen, M., Sun, Y. et al. Fine mapping of QTLs for resistance to Phytophthora nicotianae in flue-cured tobacco using a high-density genetic map. Mol Breeding 40, 45 (2020). https://doi.org/10.1007/s11032-020-01126-8
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DOI: https://doi.org/10.1007/s11032-020-01126-8