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Identification and fine mapping of a major QTL, qHD19, that plays pleiotropic roles in regulating the heading date in rice

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Abstract

As a food consumed by more than half of the world’s population, rice (Oryza sativa) has always been a hot spot in plant science research. The three most important agronomic traits of rice, the yield, plant height, and flowering time, are controlled by many quantitative trait locus (QTLs). In this study, a newly identified QTL, qHD19, was found to be controlled by a single recessive gene. Using two populations with chromosome segment substitutions, qHD19 was narrowed to a 22.5-kb region containing three putative genes, one of which encoded a ZOS5-02-C2H2 zinc finger protein. This gene was regarded as the qHD19 candidate. Further analysis showed that the amino acid sequence encoded by the qHD19 gene included two amino acid mutations, (84): lysine (L) replaced by phenylalanine (F), and (169): alanine (A) replaced valine (V), and these mutations are expected to significantly alter the functions of the protein. Additionally, the CSSL87 and CSSL88 lines containing the qHD19 gene not only exhibited a shorter plant height but also exhibited a higher yield, which showed that the qHD19 gene presents good application prospects in rice breeding. Taken together, these data indicate that qHD19 probably plays an important role in the signaling network of photoperiodic flowering as well as the regulation of plant height and yield potential.

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Abbreviations

CSSLs :

Chromosome segment substitution lines

QTLs :

Quantitative trait locus

SSR :

Simple sequence repeat

INDEL :

Insertion/deletion

ORF :

Open reading frame

BAC :

Bacterial artificial chromosome

PAC :

P1-derived artificial chromosome

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Author’s contribution statement

DY drafted the manuscript. DY, XY, XZ, CC, and NY contributed to the data analysis. DY participated in the design of the study and the interpretation of the results and wrote and edited the manuscript.

Funding

This work was supported by the Special Fund for Agro-scientific Research in the Public Interest of Fujian Province (No. 2017R1021-5, 2017R1021-2, 2016R1020-13, 2016R1020-7), the Youth Technology Innovation Team of the Fujian Academy of Agricultural Sciences (No. STIT2017-3-3), the Fujian Provincial Natural Science Foundation of China (No. 2019J01102), the General Project of the Fujian Academy of Agricultural Sciences (No. A2017-13), the Science and Technology Innovation Project of the Fujian Academy of Agricultural Sciences (No. PC2018-2), and the Free Exploration Project of the Fujian Academy of Agricultural Sciences (No. AA2018-21).

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Correspondence to Dewei Yang or Xinfu Ye.

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Yang, D., Cheng, C., Zheng, X. et al. Identification and fine mapping of a major QTL, qHD19, that plays pleiotropic roles in regulating the heading date in rice. Mol Breeding 40, 30 (2020). https://doi.org/10.1007/s11032-020-1109-x

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