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Characterization and fine-mapping of qHd2-1, a minor quantitative locus that affects heading date under long-day conditions in rice (Oryza sativa L.)

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Abstract

Heading date, a complex quantitative trait, determines regional adaptability and is associated with grain yield and quality in rice. In this study, we characterized qHd2-1, a minor-effect quantitative locus (QTL) for promoting heading date (by 3–4 days) under long-day (LD) conditions, but not short-day (SD) conditions. The qHd2-1 locus was detected in single segment substitution line (SSSL) N22-1, which was developed using indica 9311 as the donor and japonica Nipponbare (NP) as the recipient. A set of substitution lines were developed from a segregating population derived from a cross between N22-1 and NP. Based on the genotypic identification and phenotypic evaluation of the target substitution lines, qHd2-1 was narrowed down to a 105-kb region between STS2-20 and STS2-22 on the short arm of chromosome 2. Furthermore, qHd2-1 was found to function by upregulating the florigen genes Hd3a and RFT1 during floral induction under LD conditions. Field tests showed that qHd2-1 promotion of heading date affects grain yield‑related agronomic traits, such as plant height, panicle number per plant, and flag leaf size, as well as thousand-grain weight and grain width. Taken together, these findings establish a foundation for cloning this minor-effect QTL involved in heading date, and qHd2-1 could be of great significance in rice breeding to allow the fine-tuning of heading date in rice cultivars.

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Funding

This study was supported by the National Natural Science Foundation of China (Grant Nos. 31771743 and 31971913), the Postdoctoral Science Foundation of China (Grant No. 2018M632395), and the Government of Jiangsu Province (BE2018357, PAPD, YCSL201904, and PL201903).

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  1. Zuopeng Xu and Zhiai Chen contributed equally to this work.

Authors and Affiliations

  1. Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture, Yangzhou University, Yangzhou, 225009, China

    Zuopeng Xu, Zhiai Chen, Ruixuan Wang, Yixu Miao, Hailin Gao, Shuzhu Tang, Honggen Zhang & Qiaoquan Liu

  2. Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/ Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China

    Zuopeng Xu, Shuzhu Tang, Honggen Zhang & Qiaoquan Liu

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  1. Zuopeng Xu
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  2. Zhiai Chen
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  3. Ruixuan Wang
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  4. Yixu Miao
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Contributions

Q.L. and S.T. conceived the study; Z.X., Z.C., M.Y., H.G., R.W., and H.Z. performed the experiments and analyzed the data; Q.L. reviewed and edited the article; Z.X. and H.Z. wrote the article.

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Correspondence to Honggen Zhang or Qiaoquan Liu.

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Xu, Z., Chen, Z., Wang, R. et al. Characterization and fine-mapping of qHd2-1, a minor quantitative locus that affects heading date under long-day conditions in rice (Oryza sativa L.). Mol Breeding 40, 27 (2020). https://doi.org/10.1007/s11032-020-1107-z

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