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Reverse Micelle Hollow Fiber Liquid-Phase Microextraction Coupled with HPLC for the Determination of Q-Markers of Anthraquinones in Rhubarb and Their Plasma Protein Binding Rates

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Abstract

A three-phase hollow fiber liquid-phase microextraction based on reversed micellar supramolecule was developed and employed for the determination of quality markers of three anthraquinones in Rhubarb and their plasma protein binding rates. Moreover, the extraction mechanism of reversed micellar supramolecule and determination mechanism of plasma protein binding rate by the proposed method are both explored. In this procedure, n-nonanol was impregnated in the wall pores and reverse micelle of tetrabutylammonium chloride/n-nonanol filled in lumen of hollow fibers, which were covered with a thin salt film and used for the microextraction of target compounds prior to HPLC analysis. Important extraction parameters including extraction solvent type, reversed micellar supramolecule type and concentration, sample phase pH, salt concentration, stirring rate, extraction time, and binding time between active compound and plasma protein were investigated. Under the optimized conditions, limits of detection were 0.1–0.4 ng/mL with enrichment factors in the range of 54.2–100.7. The linearities were 0.5–500 ng/mL with r2 ≥ 0.9838. Satisfactory accuracies (relative recoveries 95.7–98.3%) were also obtained. The average plasma protein binding rates of rhein, chrysophenol, and physcion were 96.7%, 41.8%, and 46.7%, respectively. The results showed that the proposed procedure can not only extract and concentrate anthraquinones in Rhubarb, but also determine their plasma protein binding rates.

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Abbreviations

ACTPPBR :

Active compound-total plasma protein binding rate

EE :

Extraction efficiency

EF :

Enrichment factor

HF:

Hollow fiber

HF-LPME:

Hollow fiber liquid-phase microextraction

HPC:

Hexadecylpyridinium chloride monohydrate

LPME:

Liquid-phase microextraction

MTAC:

Methyltrioctylammonium chloride

Q-marker:

Quality marker

RM-HF-LPME:

Reverse micelle hollow fiber liquid-phase microextraction

RMS:

Reversed micellar supramolecule

TBAC:

Tetrabutylammonium chloride

TCM:

Traditional Chinese medicine

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (Grant No. 81973287) and Natural Science Foundation of Shanxi Province (Grant No. 201701D121145).

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Authors and Affiliations

  1. School of Pharmacy, Shanxi Medical University, Taiyuan, 030001, People’s Republic of China

    Shuang Hu, Shu-mei Zhang, Chun-lu Wang, Xiao-ping Bi & Xiao-hong Bai

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  1. Shuang Hu
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  2. Shu-mei Zhang
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  3. Chun-lu Wang
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Correspondence to Xiao-ping Bi or Xiao-hong Bai.

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Hu, S., Zhang, Sm., Wang, Cl. et al. Reverse Micelle Hollow Fiber Liquid-Phase Microextraction Coupled with HPLC for the Determination of Q-Markers of Anthraquinones in Rhubarb and Their Plasma Protein Binding Rates. Chromatographia 83, 757–766 (2020). https://doi.org/10.1007/s10337-020-03888-x

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