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Methylation Changes in Response to Hypoxic Stress in Wheat Regulated by Methyltransferases

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Abstract

Waterlogging is a global abiotic stress that seriously restricts the growth and yield of wheat. DNA methylation is the most common epigenetic modification and plays an important role in plant responses to adverse conditions. In this study, the methylation-sensitive amplification polymorphism (MSAP) sequencing approach was used to detect the methylation levels and pattern changes under hypoxia stress in different waterlogging tolerance wheat (Triticum aestivum L.) genotypes. Methyltransferase gene expression and protein levels were higher in the tolerant wheat genotype. Although demethylation occurred predominantly in both wheat genotypes, waterlogging-related genes, such as ERF1, ACC1, and CKX2.3, were significantly upregulated only in the tolerant genotype by demethylation, whereas other genes, such as RGA2, were significantly downregulated only in the sensitive genotype. N6-Methyladenosine (m6A) content decreased significantly in the leaves of the sensitive genotype but remained unchanged in the tolerant genotype. Our results showed that both DNA and RNA methylations play a regulatory role in wheat response to waterlogging stress.

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Funding

This work was supported by National Nature Science Foundation of China (project no. 31471496).

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Authors and Affiliations

  1. Hubei Collaborative Innovation Center for Grain Industry, School of Agriculture, Yangtze University, Jingzhou, PR China

    R. Pan, Y. H. Xu, L. Xu, W. Jiang, Q. Wang & W. Y. Zhang

  2. Tasmanian Institute of Agriculture, University of Tasmania, Private Bag 1375, TAS 7250, Prospect, Australia

    M. X. Zhou

Authors
  1. R. Pan
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  2. Y. H. Xu
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  3. L. Xu
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  4. M. X. Zhou
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Contributions

W.Y.Z. initiated the project; R.P., W.J. and Q.W. performed the experiments; W.Y.Z., R.P., Y.H.X., L.X. and M.X.Z. analyzed the data. All the authors discussed the results and approved the manuscript.

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Correspondence to W. Y. Zhang.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants as objects of research.

Additional information

Abbreviations: ACC1—acetyl-coA carboxylase 1; ERF1—ethylene response factor 1; MSAP—methylation sensitive amplification polymorphism; m6A—N6-methyladenosine.

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Pan, R., Xu, Y.H., Xu, L. et al. Methylation Changes in Response to Hypoxic Stress in Wheat Regulated by Methyltransferases. Russ J Plant Physiol 67, 323–333 (2020). https://doi.org/10.1134/S1021443720020120

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  • DOI: https://doi.org/10.1134/S1021443720020120

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