Skip to main content
SpringerLink
Log in

Effect of Short-Term Treatment of Roots with Melatonin on Photosynthesis of Cucumber Leaves

  • RESEARCH PAPERS
  • Published:
Russian Journal of Plant Physiology Aims and scope Submit manuscript

Abstract

Effect of a short-term (15, 30, 60, and 120 min) root treatment with melatonin (0.1 pM and 1.0 µM) on physiological parameters of the leaf was studied in 21-day-old cucumber (Cucumis sativus L.) plants, early cv. Izyashchnyi, grown on Murashige-Skoog nutrient medium. Experimental data concerning kinetics of melatonin influence have shown that the duration of the aftereffect necessary for activation of photosynthesis, transpiration, and stomatal conductance of cucumber leaves decreased when the concentration of the exogenous melatonin used for root treatment rose. A similar pattern was observed in respect to intensification of lipids’ peroxidation, content of proline, and activation of guaiacol-dependent peroxidase (ЕС 1.11.1.7). Under the effect of melatonin, antiradical activity in the leaf decreased in 120 min, which was preceded by a reduction in the content of total flavonoids. Regulation in activity of antioxidant enzymes superoxide dismutase (ЕС 1.15.1.1) and catalase (ЕС 1.11.1.6) was more intricate. Possible mechanisms of the influence of melatonin on parameters of photosynthesis in cucumber leaves are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.

Similar content being viewed by others

REFERENCES

  1. Arnao, M.B. and Hernandez-Ruiz, J., Melatonin and its relationship to plant hormones, Ann. Bot., 2018, vol. 121, p. 195. https://doi.org/10.1093/aob/mcx114

    Article  CAS  PubMed  Google Scholar 

  2. Zheng, X., Tan, D.X., Allan, A.C., Zuo, B., Zhao, Y., Reiter, R.J., Wang, L., Wang, Z., Guo, Y., Zhou, J., Shan, D., Li, Q., Han, Z., and Kong, J., Chloroplastic biosynthesis of melatonin and its involvement in protection of plants from salt stress, Sci. Rep., 2017, vol. 7: e41236. https://doi.org/10.1038/srep41236

    Article  CAS  Google Scholar 

  3. Sharif, R., Xie, C., Zhang, H., Arnao, M.B., Ali, M., Ali, Q., Muhammad, I., Shalmani, A., Nawaz, M.A., Chen, P., and Li, Y., Melatonin and its effects on plant systems, Molecules, 2018, vol. 23, p. 2352. https://doi.org/10.3390/molecules23092352

    Article  CAS  PubMed Central  Google Scholar 

  4. Wei, J., Li, D.X., Zhang, J.R., Shan, C., Rengel, Z., Song, Z.B., and Chen, Q., Phytomelatonin receptor PMTR1-mediated signaling regulates stomatal closure in Arabidopsis thaliana,J. Pineal Res., 2018, vol. 65: e12500. https://doi.org/10.1111/jpi.12500

    Article  CAS  PubMed  Google Scholar 

  5. Zhang, N., Zhang, H., Sun, Q., Cao, Y., Li, X., Zhao, B., Wu, P., and Guo, Y., Proteomic analysis reveals a role of melatonin in promoting cucumber seed germination under high salinity by regulating energy production, Sci. Rep., 2017, vol. 7: 503. https://doi.org/10.1038/s41598-017-00566-1

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Galano, A., Medina, M.E., and Tan, D.X., Melatonin and its metabolite as copper chelating agents and their role in inhibiting oxidative stress: a physiochemical analysis, J. Pineal Res., 2015, vol. 58, p. 107. https://doi.org/10.1111/jpi.12196

    Article  CAS  PubMed  Google Scholar 

  7. Zhang, N., Sun, Q., Zhang, H., Cao, Y., Weeda, S., Ren, S., and Guo, Y.D., Roles of melatonin in abiotic stress resistance in plants, J. Exp. Bot., 2015, vol. 66, p. 647. https://doi.org/10.1093/jxb/eru336

    Article  CAS  PubMed  Google Scholar 

  8. Wang, L.Y., Liu, J.L., Wang, W.X., and Sun, Y., Exogenous melatonin improves growth and photosynthetic capacity of cucumber under salinity-induced stress, Photosynthetica, 2016, vol. 54, p. 19. https://doi.org/10.1007/s11099-015-0140-3

    Article  CAS  Google Scholar 

  9. Kholodova, V.P., Vasil’ev, S.V., Efimova, M.V., Voronin, P.Yu., Rakhmankulova, Z.F., Danilova, E.Yu., and Kuznetsov, Vl.V., Exogenous melatonin protects canola plants from toxicity of excessive copper, Russ. J. Plant Physiol., 2018, vol. 65, p. 882. https://doi.org/10.1134/S1021443718060080

    Article  CAS  Google Scholar 

  10. Golovatskaya, I.F., Boyko, E.V., and Karnachuk, R.A., Role of melatonin in the regulation of IAA-dependent plant reaction in different lighting conditions, Vestn. Tomsk. Gos. Univ., Ser. Biol., 2017, no. 37, p. 144. https://doi.org/10.17223/19988591/37/8

  11. Weeda, S., Zhang, N., Zhao, X., Ndip, G., Guo, Y., Buck, G.A., Fu, C., and Ren, S., Arabidopsis transcriptome analysis reveals key roles of melatonin in plant defense systems, PLoS One, 2014, vol. 9: e93462. https://doi.org/10.1371/journal.pone.0093462

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Golovatskaya, I.F., Boyko, E.V., Vidershpan, A.N., and Laptev, N.I., Age-dependent morphophysiological changes and biochemical composition of Lactuca sativa L. plants influenced by Se and solar radiation of varying intensity, S-kh.Biol., 2018, vol. 53, no. 5, p. 1025. https://doi.org/10.15389/agrobiology.2018.5.1025rus

    Article  Google Scholar 

  13. Golovatskaya, I.F., Bender, O.G., Efimova, M.V., Boyko, E.V., Malofii, M.K., Murgan, O.K., and Plyusnin, I.N., The role of exogenous steroid phytohormones in regulating the functioning of the photosynthetic apparatus of plants, Mater. Vseros. nauchno-praktich. konf. s mezhd. uchastiem “Aktual’nye problemy kartofelevodstva: fundamental’nye i prikladnye aspekty” (Proc. All-Russia Sci.-Pract. Conf. with Int. Participation “Current Problems of Potato Growing: Fundamental and Applied Aspects”), Tomsk, 2018, p. 103.

  14. Buege, J.A. and Aust, S.D., Microsomal lipid peroxidation, Methods Enzymol., 1978, vol. 52, p. 302.

    Article  CAS  Google Scholar 

  15. Blois, M.S., Antioxidant determinations by the use of a stable free radical, Nature, 1958, vol. 181, p. 1199.

    Article  CAS  Google Scholar 

  16. Bates, L.S., Waldren, R.P., and Teare, I.D., Rapid determination of free proline for water stress studies, Plant Soil, 1973, vol. 39, p. 205.

    Article  CAS  Google Scholar 

  17. Efimova, M.V., Kolomeichuk, L.V., Boyko, E.V., Malofii, M.K., Vidershpan, A.N., Plyusnin, I.N., Golovatskaya, I.F., Murgan, O.K., and Kuznetsov, Vl.V., Physiological mechanisms of Solanum tuberosum L. plants’ tolerance to chloride salinity, Russ. J. Plant Physiol., 2018, vol. 65, p. 394. https://doi.org/10.1134/S1021443718030020

    Article  CAS  Google Scholar 

  18. Esen, A.A., Simple method for quantitative, semiquantitative, and qualitative assay of protein, Anal. Biochem., 1978, vol. 89, p. 264.

    Article  CAS  Google Scholar 

  19. Tarakhovskii, Yu.S., Kim, Yu.A., Abdrasilov, B.S., and Muzafarov, E.N., Flavonoidy: biokhimiya, biofizika, meditsina (Flavonoids: Biochemistry, Biophysics, and Medicine), Pushchino: Sunchrobook, 2013.

  20. Boyko, E.V. and Golovatskaya, I.F., Influence of pre-sowing treatment with melatonin and sodium selenite on seed germination, Mater. XIII Mezhd. simp. “Novye i netraditsionnye rasteniya i perspektivy ikh ispol’zovaniya” (Proc. XIII Int. Symp. “New and Non-Traditional Plants and Prospects for Their Use”), Moscow, 2019, p. 135.

  21. Zhang, N., Sun, Q.Q., Li, H.F., Li, X.S., Cao, Y.Y., Zhang, H.J., Li, S.T., Zhang, L., Qi, Y., Ren, S.X., Zhao, B., and Guo, Y.D., Melatonin improved anthocyanin accumulation by regulating gene expressions and resulted in high reactive oxygen species scavenging capacity in cabbage, Front. Plant Sci., 2016, vol. 7: 222. https://doi.org/10.3389/fpls.2016.00197

    Article  Google Scholar 

  22. Yang, X.L., Xu, H., Li, D., Gao, X., Li, T.L., and Wang, R., Effect of melatonin priming on photosynthetic capacity of tomato leaves under low-temperature stress, Photosynthetica, 2018, vol. 56, p. 884. https://doi.org/10.1007/s11099-017-0748-6

    Article  CAS  Google Scholar 

  23. Li, C., Tan, D.X., Liang, D., Chang, C., Jia, D., and Ma, F., Melatonin mediates the regulation of ABA metabolism, free-radical scavenging, and stomatal behavior in two Malus species under drought stress, J. Exp. Bot., 2015, vol. 66, p. 669. https://doi.org/10.1093/jxb/eru476

    Article  CAS  PubMed  Google Scholar 

  24. Yoon, Y., Kim, M., and Park, W., Foliar accumulation of melatonin applied to the roots of maize (Zea mays) seedlings, Biomolecules, 2019, vol. 9, p. 26. https://doi.org/10.3390/biom9010026

    Article  CAS  PubMed Central  Google Scholar 

  25. Li, H., Chang, J., Zheng, J., Dong, Y., Liu, Q., Yang, X., Wei, C., Zhang, Y., Ma, J., and Zhang, X., Local melatonin application induces cold tolerance in distant organs of Citrullus lanatus L. via long distance transport, Sci. Rep., 2017, vol. 7: e40858. https://doi.org/10.1038/srep40858

    Article  CAS  Google Scholar 

  26. Ye, J., Wang, S., Deng, X., Yin, L., Xiong, B., and Wang, X., Melatonin increased maize (Zea mays L.) seedling drought tolerance by alleviating drought-induced photosynthetic inhibition and oxidative damage, Acta Physiol. Plant., 2016, vol. 38, p. 48. https://doi.org/10.1007/s11738-015-2045-y

    Article  CAS  Google Scholar 

  27. Garifzyanov, A.R., Zhukov, N.N., and Ivanishchev, V.V., Formation and physiological reactions of active oxygen species in plant cells, Sovrem. Problemy Nauki i Obrazovaniya, 2011, no. 2, p. 35.

  28. Foyer, C.H., Reactive oxygen species, oxidative signaling and the regulation of photosynthesis, Environ. Exp. Bot., 2018, vol. 154, p. 134. https://doi.org/10.1016/j.envexpbot.2018.05.003

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Lee, K., Hwang, O.J., Reiter, R.J., and Back, K., Flavonoids inhibit both rice and sheep serotonin N-acetyltransferases and reduce melatonin levels in plants, J. Pineal Res., 2018, vol. 65: e12512. https://doi.org/10.1111/jpi.12512

    Article  CAS  PubMed  Google Scholar 

  30. Kuznetsov, V.V., Chloroplasts: structure and expression of the plastid genome, Russ. J. Plant Physiol., 2018, vol. 65, p. 465. https://doi.org/10.1134/S1021443718030044

    Article  Google Scholar 

Download references

Funding

This work was supported by the Program for Raising Competitive Capacity of Tomsk State University.

Author information

Authors and Affiliations

  1. National Research Tomsk State University, Tomsk, Russia

    E. V. Boyko, I. F. Golovatskaya & I. N. Plyusnin

  2. Institute of Monitoring of Climatic and Ecological Systems, Siberian Branch, Russian Academy of Sciences, Tomsk, Russia

    O. G. Bender

Authors
  1. E. V. Boyko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. F. Golovatskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. G. Bender
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. N. Plyusnin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. V. Boyko.

Ethics declarations

The authors declare that they have no conf lict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

Additional information

Translated by N. Balakshina

Abbreviations: ARA—antiradical activity; Cat—catalase; GPO—guaiacol peroxidase; POL—peroxidation of lipids; SOD—superoxide dismutase.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Boyko, E.V., Golovatskaya, I.F., Bender, O.G. et al. Effect of Short-Term Treatment of Roots with Melatonin on Photosynthesis of Cucumber Leaves. Russ J Plant Physiol 67, 351–359 (2020). https://doi.org/10.1134/S102144372002003X

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S102144372002003X

Keywords:

Search

Navigation