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Overexpression of Small Heat Shock Protein LimHSP16.45 in Arabidopsis hsp17.6II Mutant Enhances Tolerance to Abiotic Stresses

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Abstract

Organisms can produce heat shock proteins (HSPs) in response to elevated temperatures and other abiotic stresses. However, the function of HSPs, including small heat shock proteins (sHSPs), in stress tolerance is not fully explored. To improve our understanding of sHSPs, we isolated a gene of sHSPs from David lily (Lilium davidii Duchartre) called LimHSP16.45. Results reveal that LimHSP16.45 is a cytosolic class II sHSP. The HSP17.6II of Arabidopsis thaliana belongs to the HSP20 chaperone protein and is similar to Lim16.45HSP of David lily in terms of size and structure. Both genes have a small heat shock-like alpha-crystallin domain (ACD) structure. To further study the function of Lim16.45HSP, we overexpress it in Arabidopsis hsp17.6II mutant. Then, we detect the expression of LimHSP16.45 in transgenic plant under abiotic stresses and analyze the heat tolerance of transgenic plant. In addition, we measure the activity of three antioxidant enzymes (peroxidase, catalase and superoxide dismutase) and the content of soluble sugar and proline in transgenic plants under abiotic stresses.We found that transgenic plant is tolerant to heat and oxidative stresses given its increased survival rate relative to the hsp17.6II and wild type. Moreover, the content of soluble sugar and proline considerably increase in the transgenic plant. These results support the positive role of LimHSP16.45 in response to heat stress in plants. We suspect that LimHSP16.45 enhances plant tolerance to abiotic stresses by stimulating the activity of ROS-scavenging enzymes and other protective enzymes and increasing the synthesis of proline.

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ACKNOWLEDGMENTS

Thanks to Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University.

Funding

This work was financially supported by grants from the National Natural Science Foundation of China (project nos. 31770326 and 31300229), and Fundamental Research Funds for the Central Universities (project no. lzujbky-2017-149).

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Authors and Affiliations

  1. Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China

    R. Yang, G. Yu, H. Li, X. Li & C. Mu

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  1. R. Yang
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  2. G. Yu
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  3. H. Li
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  4. X. Li
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  5. C. Mu
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Contributions

Changjun Mu, Ruizhen Yang, and Xiaofeng Li designed the experiments; Ruizhen Yang and Guanzhong Yu performed most of the experiments; Haojie Li interpreted data and generated figures; Changjun Mu and Ruizhen Yang wrote the manuscript.

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Correspondence to C. Mu.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants as objects of research.

Additional information

Abbreviations: ACD—alpha-crystallin domain; APX—ascorbate peroxidase; CAT—catalase; HSGs—heat shock granules; HSP—heat shock protein; POD—peroxidase; ROSs—reactive oxygen species; sHSP—small heat shock protein; SOD—superoxide dismutase; WT—wild type.

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Yang, R., Yu, G., Li, H. et al. Overexpression of Small Heat Shock Protein LimHSP16.45 in Arabidopsis hsp17.6II Mutant Enhances Tolerance to Abiotic Stresses. Russ J Plant Physiol 67, 231–241 (2020). https://doi.org/10.1134/S102144372002017X

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