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Biotechnological Potential of the Bacillus subtilis 20 Strain

  • MOLECULAR CELL BIOLOGY
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Abstract—

Bacillus subtilis bacteria play an important role in veterinary medicine, medicine, and biotechnology, and the permanently growing demand for biotechnological products fuels the improvement of the properties of biotechnological strains. B. subtilis strains with improved characteristics may be obtained by rational design and the directed evolution technologies, or be found among newly described strains. In the course of the long-term microbiome composition studies in the Russian segment of the International Space Station, the B. subtilis 20 strain was isolated, this strain shows the capacity for rapid growth and considerable biomass accumulation, as well as increased resistance to acidification of the environment in comparison to the “terrestrial” B. subtilis 168 strain. What is more, B. subtilis 20 is hyperresistant to the DNA and protein damaging factors that are linked to the overexpression of the genes controlling DNA repair, hydrogen sulfide production, and reactive oxygen species neutralization. The described properties of B. subtilis 20 are indicative of its considerable potential as a promising producer of biologically active compounds.

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Funding

The work was supported by the Russian Science Foundation (grant no. 17-74-30030) (Table 1 and Figs. 2, 3, 5, and 6), the Fundamental Research Program for the State Academies of Sciences (grant no. АААА-А19-119010590015-8) (Fig. 4), and the Russian Academy of Sciences Programs nos. 01201367566 (Fig. 1) and 01201373016 (Table 2).

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Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia

    D. S. Karpov, M. I. Kotlov, P. G. Osipova, S. V. Kiseleva, T. A. Seregina, A. S. Mironov & V. L. Karpov

  2. Institute of Medical and Biological Problems, Russian Academy of Sciences, 123007, Moscow, Russia

    A. I. Domashin & S. V. Poddubko

  3. Fundamentals of Biotechnology Federal Research Centre, Russian Academy of Sciences, 119071, Moscow, Russia

    A. V. Goncharenko

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  1. D. S. Karpov
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  2. A. I. Domashin
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  3. M. I. Kotlov
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  4. P. G. Osipova
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  5. S. V. Kiseleva
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  6. T. A. Seregina
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  7. A. V. Goncharenko
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  8. A. S. Mironov
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  9. V. L. Karpov
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  10. S. V. Poddubko
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Correspondence to D. S. Karpov.

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The article does not contain any studies involving animals or humans performed by any of the authors.

Conflict of interest. The authors declare no conflict of interest.

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Translated by E. Martynova

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Karpov, D.S., Domashin, A.I., Kotlov, M.I. et al. Biotechnological Potential of the Bacillus subtilis 20 Strain. Mol Biol 54, 119–127 (2020). https://doi.org/10.1134/S0026893320010082

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  • DOI: https://doi.org/10.1134/S0026893320010082

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