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First report on DNA content of three species of Amorphophallus

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Abstract

The Amorphophallus genus is a perennial herb which belongs to the family Araceae. There are more than 170 species in this genus, which is widely distributed in tropical and subtropical areas. As a kind of food and medicine Amorphophallus has been used for more than 2000 years in China. Because of the high content of konjac glucomannan (KGM) and dietary fiber, it has attracted more attention worldwide. In this article, the DNA contents of A. konjac, A. albus and A. bulbifer in China, A. albus, A. paeoniifolius and A. muelleri in Indonesia were estimated by using flow cytometry. In the samples of China, the DNA contents were 12.95 ± 0.73 pg/2C in A. konjac, 10.51 ± 0.05 pg/2C in A. albus and 17.61 pg/2C in A. bulbifer, and for Indonesia, 14.16 ± 0.48 pg/2C in A. albus (flowering), 8.49 ± 0.2 pg/2C in A. paeoniifolius and 17.84 ± 1.46 pg/2C in A. muelleri were used. Interspecific variation was found significantly (P < 0.01), suggesting that DNA content might be a parameter that can be used to differentiate the species. Intraspecific variation has also been found significantly (P < 0.01), whether in the same region or between two regions. As far as we know, this is the first report on genome size estimation of the A. konjac, A. albus and A. muelleri using flow cytometry. Understanding the genome size of Amorphophallus species will help to sequence the genome and analyse the genetic diversity, evolutionary relationship and geographical variation pattern of Amorphophallus species.

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Acknowledgements

This study was supported by grants from the National Science and Technology Supporting Programme (No. 2011BAD33B03), the Fundamental Research Funds for the Central Universities (No. 2042016kf1106) and Philanthropic Project of Scientific Research of Hubei (No. 2012DBA11001). Thanks to Li Ye and Sheng Jiajing for their help in the experiment process.

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Authors and Affiliations

  1. State Key Laboratory of Hybrid Rice, Lotus Engineering Research Centre of Hubei Province, College of Life Sciences, Wuhan University, Wuhan, 430070, Hubei, People’s Republic of China

    CHUFENG ZHAO, ERXI LIU, YING DIAO & ZHONGLI HU

  2. Faculty of Mathematics and Natural Sciences, Department of Biology, Brawijaya University, Jl.Veteran, Malang, 65145, Indonesia

    NUNUNG HARIJATI

  3. Institute of Konjac, Enshi Academy of Agricultural Sciences, Enshi, 445000, Hubei, People’s Republic of China

    ERXI LIU

  4. School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan, 430070, People’s Republic of China

    SURONG JIN

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  1. CHUFENG ZHAO
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Correspondence to ZHONGLI HU.

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Corresponding editor: Manoj Prasad

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ZHAO, C., HARIJATI, N., LIU, E. et al. First report on DNA content of three species of Amorphophallus. J Genet 99, 36 (2020). https://doi.org/10.1007/s12041-020-01199-6

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  • DOI: https://doi.org/10.1007/s12041-020-01199-6

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