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Gamma radiation-induced DNA damage in adults of the coffee berry borer, Hypothenemus hampei Ferrari (Coleoptera: Curculionidae)

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Abstract

Using comet assay DNA damage in the cells of Hypothenemus hampei adults was assessed following irradiation from a 60Co source at gamma radiation doses 10, 20, 40, 80, 160, 320, 640, 1600 and 3200 Gy. The survival of exposed adults at different interval of time was assessed for the same doses of radiation. Radiation-induced DNA damage measured by increased strand breaks at higher doses was significantly different from intact cells found in control, where the increase in damage was dose-dependent. The survival of the exposed adults was dose-dependent. Doses of 1600 and 3200 Gy resulted in 100% mortality within 15 days of irradiation. The absence of significant radiation induced changes in DNA damage and survival of adults at lower doses indicates greater tolerance to radiation. The study provides useful information about the radiation sensitivity of H. hampei to develop an effective method for the control of the borer in harvested coffee.

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Acknowledgements

This work was supported by the grants of Board of Research in Nuclear Sciences (BRNS), Department of Atomic Energy (DAE), Bhabha Atomic Research Centre (BARC), Mumbai (No2013/35/1/BRNS/15 Dated 01/04/2013). We are thankful to Centre for Application of Radioisotopes and Radiation Technology (CARRT), Mangalore University, Karnataka, India for providing radiation facility and Dr. Swaroop K for his help in the radiation of samples.

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  1. Insect Science Laboratory, Department of Zoology, Bangalore University, Bengaluru, 560056, India

    R. Kiran & Melally G. Venkatesha

  2. Department of Applied Zoology, Mangalore University, Mangaluru, 574199, India

    K. Bhasker Shenoy

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Correspondence to Melally G. Venkatesha.

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Kiran, R., Shenoy, K.B. & Venkatesha, M.G. Gamma radiation-induced DNA damage in adults of the coffee berry borer, Hypothenemus hampei Ferrari (Coleoptera: Curculionidae). Int J Trop Insect Sci 40, 773–779 (2020). https://doi.org/10.1007/s42690-020-00130-x

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