Abstract
Identifying resistance to plant-parasitic nematodes in yams is a major challenge in yam breeding programmes due partly to insufficient planting material (tubers) for evaluation. Susceptible yam cultivar, TDr 93-31 was inoculated with 0 (control), 200, 500, 1000 and 2000 eggs/individuals each of Meloidogyne incognita, Scutellonema bradys and Pratylenchus brachyurus. Vine cuttings of two cultivars each of Dioscorea rotundata, D. alata and D. dumetorum were compared to conventional minisett method in a second experiment. Planted vines and minisetts were inoculated with 500 and 5000 eggs/individuals respectively. Data were collected on percentage vine survival, fresh root and tuber weight, number of tubers and nematode population and damage. Vine survival was not significantly different between control and inoculated vines. Maximum damage rating (>60%) was observed with initial inoculum of 1000 eggs of M. incognita and 200 individuals of S. bradys and P. brachyurus. The two cultivars of D. alata and D. rotundata developed visible symptoms and supported nematode reproduction while the D. dumetorum cultivars did not. The reaction of yam cultivars planted as vines was consistent with results from conventional minisett method. The lowest inoculum of 200 egg/juveniles was sufficient to elicit a reaction from vines in the vertical sack system.
Similar content being viewed by others
References
Acosta N, Ayala AL (1975) Pathogenicity of Pratylenchus coffeae, Scutellonema bradys, Meloidogyne incognita, and Rotylenchus reniformis on Dioscorea rotundata. Journal of Nematology 7(1):1–6
Acosta N, Ayala AL (1976) Effect of Pratylenchus coffeae and Scutellonema bradys alone and in combination on guinea yam (Dioscorea rotundata). Journal of Nematology 8:315–317
Adesiyan SO, Odihirin R, Adeniji MO (1975) Economic losses caused by the yam nematode, Scutellonema bradys in Nigeria. Plant Disease Reporter 59:477–480
Atu UG, Odurukwe SO, Ogbuji RO (1983) Root-knot nematode damage to Dioscorea rotundata. Plant Disease 67:814–815
Ayankanmi TG, Agele SO (2010) Effects of genotype, root--promoting substances and planting media on yam (Dioscorea rotundata Poir) vine cuttings for mini tuber production. Advances in Environmental Biology 2010:353–360
Hussey RS, Barker KR (1973) A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Disease Reporter 57:1025–1028
Bridge J (1973) Nematodes as pests of yams in Nigeria. Mededelingen Faculteit. Landbouwwetenschappen Gent 38:841–852
Bridge J (1982). Nematodes of yams. In: Miege J, Lyonga SN , eds. Yams. Ignames. New York, USA: Oxford University Press, pp. 253-264.
Bridge J, Coyne D, Kwoseh CK (2005). Nematode Parasites of Tropical Root and Tuber Crops (excluding potatoes). In: Luc M., Sikora R. A., Bridge J. (Eds.). Plant parasitic nematodes in subtropical and tropical agriculture. 2nd Edition, CAB International Publishing. pp. 221-258
Castillo P, Vovlas N (2007) Pratylenchus, (Nematoda, Pratylenchidae): diagnosis, biology, pathogenicity and management. Nematology Monographs and Perspectives 6:529
Claudius-Cole AO (2005). Cover crops in the management of Meloidogyne incognita and Scutellonema bradys on yams (Dioscorea sp.). Ph.D. thesis, Department of Crop Protection and Environmental Biology, University of Ibadan, Ibadan. Nigeria.
Claudius-Cole AO, Coyne D, Kenyon L, Morse S, Ayodele M, McNamara N (2004) Healthy seed yam production systems in Nigeria. First West and Central African Nematology Conference, Book of Abstracts, 8-11 November, 2004. Douala, Cameroon, p 23
Claudius-Cole AO, Kenyon L, Coyne DL (2017) Effect of pre-plant treatments of yam (Dioscorea rotundata) setts on the production of healthy seed yam, seed yam storage and consecutive ware tuber production. Journal of Agriculture and Rural Development in the Tropics and Subtropics 118(2):297–306
Coyne DL, HAL T, Maslen NR (2003) Plant-parasitic nematodes associated with root and tuber crops in Uganda. African Plant Protection 9:87–98
Coyne DL, Tchabi A, Baimey H, Labuschagne N, Rotifa I (2006) Distribution and prevalence of nematodes (Scutellonema bradys and Meloidogyne spp.) on marketed yam (Dioscorea spp.) in West Africa. Field Crops Research 96(1):142–150
Coyne D, Nicol J, Claudius-Cole A (2007) Practical Plant Nematology: Field and Laboratory Guide. IITA, Ibadan, Nigeria, 82 pp
Ettien JB, Sorho F, Brahima K (2013) Screening of new yam clones (D. alata and D. rotundata) in nematode prone ecology of guinea savanna zone in West Africa. Journal of Applied Biosciences 61:4540–4550
Hooper DJ (1990) Extraction and processing of plant and soil nematodes. In: Luc M, Sikora R, Bridge J (eds) Plant Parasitic Nematodes in Subtropical and Tropical Agriculture. CAB International, Wallingford, UK, pp 45–68
Kwoseh CK, Plowright RA, Bridge J, Asiedu R (2007) Search for Scutellonema bradys resistance in yams (Dioscorea spp.). Journal of Science and Technology Ghana 27:23–42
Mudiope J, Speijer PR, Maslen NR, Adipala E (2001). Pratylenchus, the dominant genus affecting yam (Dioscorea spp.) in Uganda. Proceedings of the 7th Triennial Symposium of the International Society for Tropical Root Crops- Africa Branch, 11-17 October 1988, Cotonou, Benin. pp. 637-638
Mudiope J, Coyne DL, Adipala E, Sikora RA (2004) Pathogenicity of the lesion nematode, Pratylenchus sudanensis (Loof and Yassin) in pots. In: Proceeding of the 6th Africa Crop Science Conference, 12-17 October 2003. Kenya, Nairobi
Nwauzor EC, Fawole B (1981) Root-knot nematodes on yams (Dioscorea spp.) in eastern Nigeria. Proceeding of the 3rd Research Planning Conference on Root-knot nematodes, Meloidogyne spp. Region IV and V, Ibadan, Nigeria, pp 161–167
Oluwatayo JI, Asiedu R, Adesiyan SO (2010) Screening plant materials for resistance to the yam nematode (Scutellonema bradys). African Journal of Root and Tuber Crops 8(2):34
Otoo E, Anyakanmi TG, Kikuno H, Asiedu R (2016). In Vivo Yam (Dioscorea spp.) Vine Multiplication Technique: The Plausible Solution to Seed Yam Generation Menace. Journal of Agricultural Science 8(2): 88.
Plowright R, Kwoseh C. (2009). Identification of resistance to major nematode pests of yams.(Dioscorea) in West Africa. Final Technical Report. http://agris.fao.org/agris-search/search.do?recordID=GB2012104399
SAS [Statistical Analysis System]. (2014) SAS Institute. Inc, Cary, NC, USA
Shiwachi H, Kikuno H, Asiedu R (2005) Mini Tuber Production Using Yam (Dioscorea rotundata)Vines. Tropical Science 45(4):163–169
Siddiqi MR (2000) Tylenchida parasites of plants and insects (2nd edition). CAB International, Wallingford, UK, 833 p
Talwana LAH, Speijer PR, Adipala E, Maslen NR (1996) Evaluation of cassava for reaction to root-knot nematodes (Meloidogyne spp.) in Uganda. African Journal of Plant Protection 6:125–134
Wood TG, Smith RW, Johnson RA, Komolafe PO (1980) Termite’s damage and crop loss studies in Nigeria- pre-harvest losses to yam due to termites and other soil pests. Tropical Pest Management 26:355–370
Acknowledgments
The authors gratefully acknowledge the support from the YIIFSWA Project, funded by the Bill and Melinda Gates Foundation through the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria and from the Yam Breeding Unit of the International Institute of Tropical Agriculture, IITA, Ibadan.
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Section Editor: Diana Fernandez
Rights and permissions
About this article
Cite this article
Claudius-Cole, A.O., Omotayo, T.O. & Montes, A.L. Nodal vine cutting technique for assessing nematode resistance in yams. Trop. plant pathol. 45, 56–63 (2020). https://doi.org/10.1007/s40858-019-00325-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s40858-019-00325-9