Abstract
Pseudomonas aeruginosa, commonly found in environments, can cause chronic lung disease in immunocompromised patients. In previous study, an aerobic desaturase (DesB) in P. aeruginosa exerted considerable effects on virulence factor production. The objective of this study was to analyze the role of DesB on the virulence traits of P. aeruginosa in the host. For the in vitro experiments, cells and supernatants from wild-type (WT) P. aeruginosa and its desB mutant were collected. The diluted cells were added to the A549 cell monolayer in order to determine cell viability, invasion ability, and/or immune response. For the in vivo experiments, 6-week-old ICR mice were infected with 6–7 log CFU bacterial cells using endotracheal intubation. The ratio of lung weight to body weight and survival rate of each bacterial strain in the lung were measured. The histopathology of lung tissue was also studied. desB mutants exhibited lower cytotoxicity in A549 cells. In addition, more pro-inflammatory cytokines and chemokines were present in desB mutant-treated. In the lungs of mouse model, WT survived longer than desB mutant, and the WT migrated from the lung to the liver and spleen. The results suggest that P. aeruginosa DesB affects the pathogenicity of the organism in the host.
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This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) and funded by the Ministry of Science, ICT and Future Planning (2012R1A1A1014562).
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All animal procedures were approved by the Animal Ethical Committee of Korea University Institutional Animal Care and Use Committee (KUIACUC-2017-45).
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Supplementary figure 1
Cytotoxicity of P. aeruginosa WT or desB mutant on A549-cells; Supernatant diluted to 1/2, 1/4, 1/8, and 1/16 (A), Bacterial culture diluted to 1/10, 1/20, 1/40, and 1/80 (B). (DOCX 66 kb)
Supplementary figure 2
The number of P. aeruginosa WT and desB mutant remaining in the infected lungs; H6: 6 h after inhalation, H11: 11 h after inhalation. (DOCX 51 kb)
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Ha, J., Kim, S., Lee, J. et al. The role of Pseudomonas aeruginosa DesB in pathogen-host interaction. Int Microbiol 23, 549–555 (2020). https://doi.org/10.1007/s10123-020-00130-4
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DOI: https://doi.org/10.1007/s10123-020-00130-4