Abstract
Stripe rust, which is caused by Puccinia striiformis f. sp. Tritici (Pst), is one of the most destructive wheat (Triticum aestivum L.) diseases worldwide. To control stripe rust, the best strategy is breeding and growing novel resistant cultivars. Qubaichun (QBC), a Tibetan wheat landrace, displays near-immune resistance to wheat stripe rust in western China. Previously, our studies have shown that the stripe rust resistance of QBC is controlled by a dominant gene at the seedling stage and two independent genes at the adult-plant stage. These two genes comprise an all-stage resistance (ASR) gene and a durable adult resistance gene, which was identified as Yr18. The unknown ASR gene is temporarily named Yrqbc. To map this gene, a segregating population of QBC × Chinese Spring (CS) was generated. SSR analysis, BSR-seq and Infinium 660 K iSelect SNP genotyping were successively performed. The results show that Yrqbc finely mapped to a 5.1 cM genetic interval between molecular markers A009200 and A009192, and the genetic distance to the marker A009200 was 0.1 cM. Furthermore, Yrqbc was confirmed to be Yr5 by sequencing. Diagnostic markers are used for detection Yr5 and Yr18 in 323 new cultivars (lines) worldwide. The results show that 27 cultivars (lines) carry the durable adult resistance gene Yr18, and only one material carrying the ASR resistance gene Yr5. In order to analyse the effectiveness and practicality of transferring the pyramided of Yr18 and Yr5, these two stripe rust resistance genes were introduced into elite cultivars which lost resistance. Field test results verified that the combination of Yr5 and Yr18 could provide effective resistance to stripe rust for the whole life of wheat, which provided a genetic foundation for the near immunity of elite cultivars. As a result, this Tibetan landrace could be used for developing high-level, durable resistant wheat cultivars.
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Acknowledgements
We thank Tibet Academy of Agriculture and Animal Husbandry Sciences for providing Qubaichun seeds. The KASP assay was conducted by China Golden Marker (Beijing) Biotech Co., Ltd. Thanks to Professor Zhiyong Liu from Institute of genetics, CAS for BSR-seq data analysis.
Funding
This work was supported by grants from the National Natural Science Foundation of China (Grant No. 31671679) and Youth Innovation Promotion Association, CAS (Grant No. 2018403).
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T-W coordinated the project, conceived and designed experiments. B-F conducted the bioinformatics work, generated and analyzed data, and edits the manuscript. Z-B X and F-X L collected the samples; F-W, G-S J and Q-Z performed the laboratory work. All authors read and approved the final manuscript.
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10722_2020_938_MOESM1_ESM.pptx
Supplementary file1 (PPTX 506 kb) Supplementary Figure 1 The integrated genetic linkage map of stripe rust resistance gene and its co-segregated markers on 2BL. Supplementary Figure 2 The ASR gene in QBC was identified by Yr5 functional marker. The amplified fragment size of PCR was 1281 bp. Material 1-7 was a randomly selected F2 generation of QBC × CM28 with IT of "0", Material 8 was a cultivar containing Yr5 gene, Material 9 was QBC, Material 10 was CS. The results showed that the ASR gene in QBC was Yr5. Supplementary Figure 3 The analysis results of BSR-seq, SNPs are mainly concentrated in the 633~653 Mb, 673~693 Mb and 723~763 Mb regions of 2BL
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Cao, J., Xu, Z., Fan, X. et al. Genetic mapping and utilization analysis of stripe rust resistance genes in a Tibetan wheat (Triticum aestivum L.) landrace Qubaichun. Genet Resour Crop Evol 67, 1765–1775 (2020). https://doi.org/10.1007/s10722-020-00938-z
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DOI: https://doi.org/10.1007/s10722-020-00938-z