Abstract
In this study, using PCR-RFLP molecular identification methods, the species composition was determined in populations of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) which were collected from greenhouses located in the Akdeniz, Aydıncık, Bozyazı, Erdemli, Gülnar, Silifke, and Tarsus districts of Mersin Province. For this purpose, adult whiteflies were collected from the greenhouses during the spring and autumn growing seasons of 2016 and 2017. In order to determine the species status in the off-season, samples were also collected from field crops around greenhouses during summer 2016. In all, 219, 195, and 129 females were analyzed from samples collected in spring, autumn, and off-seasons, respectively. According to the results of analysis, the species status of B. tabaci did not vary depending on location and season. Overall, MEAM1 and MED (with subgroups Q1 and Q2) species were found. The abundance rates of MEAM1 species were found to be very high in all locations, with values of 97.7% and 99.5% during spring and autumn, respectively. Similar to the greenhouse-collected populations, the MEAM1 species whiteflies collected from open field crops during the off-season were found to have a higher abundance rate (99.2%). The highest abundance rate for MED species (12.8%) was determined in Erdemli district during spring. The results of this study indicate that B. tabaci belonging to the MEAM1 is the most dominant species in greenhouses and open fields (off-season) of Mersin, Turkey, in both spring and autumn, and these results are also discussed aiming at contributing to B. tabaci management in greenhouses.
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References
Bayhan, E., Ulusoy, M. R., & Brown, J. K. (2006). Host range, distribution, and natural enemies of Bemisia tabaci ‘B biotype’ (Hemiptera: Aleyrodidae) in Turkey. Journal of Pest Science, 79, 233–240.
Bedford, I., Briddon, R. W., Brown, J. K., Rosell, R. C., & Markham, P. G. (1994). Geminivirus transmission and biological characterisation of Bemisia tabaci (Gennadius) biotypes from different geographic regions. Annals of Applied Biology, 125, 311–325.
Bello, V. H., Watanabe, L. F. M., Santos, B. R., Marubayashi, J. M., Yuki, V. A., De Marchi, B. R., et al. (2019). Evidence for increased efficiency of virus transmission by populations of Mediterranean species of Bemisia tabaci with high Hamiltonella prevalence. Phytoparasitica, 47(2), 293–300.
Boykin, L. M. (2014). Bemisia tabaci nomenclature: Lessons learned. Pest Management Science, 70, 1454–1459.
Brown, J., & Idris, A. (2005). Genetic differentiation of whitefly Bemisia tabaci mitochondrial cytochrome oxidase I, and phylogeographic concordance with the coat protein of the plant virus genus Begomovirus. Annals of the Entomological Society of America, 98, 827–837.
Brown, J. K., Frohlich, D., & Rosell, R. (1995). The sweetpotato silverleaf whiteflies: biotypes of Bemisia tabaci (Genn.), or a species complex? Annual Review of Entomology, 40, 511–534.
Castle, S. J., Palumbo, J. C., Prabhaker, N., Horowitz, A. R., & Denholm, I. (2010). Ecological determinants of Bemisia tabaci resistance to insecticides. In P. A. Stansly & S. E. Naranjo (Eds.), Bemisia, bionomics and management of a global pest (pp. 423–465). New York: Springer+Business.
Cavalieri, V., Manglli, A., Tiberini, A., Tomassoli, L., & Rapisarda, C. (2014). Rapid identification of Trialeurodes vaporariorum, Bemisia tabaci (MEAM1 and MED) and tomato-infecting criniviruses in whiteflies and in tomato leaves by real-time reverse transcription-PCR assay. Bulletin of Insectology, 67, 219–225.
Chiel, E., Gottlieb, Y., Zchori-Fein, E., Mozes-Daube, N., Katzir, N., Inbar, M., & Ghanim, M. (2007). Biotype-dependent secondary symbiont communities in sympatric populations of Bemisia tabaci. Bulletin of Entomological Research, 97, 407–413.
Chu, D., Hu, X., Gao, C., Zhao, H., Nichols, R. L., & Li, X. (2012). Use of mitochondrial cytochrome oxidase I polymerase chain reaction-restriction fragment length polymorphism for identifying subclades of Bemisia tabaci Mediterranean group. Journal of Economic Entomology, 105, 242–251.
Costa, H. S., Brown, J. K., Sivasupramaniam, S., & Bird, J. (1993). Regional distribution, insecticide resistance, and reciprocal crosses between the ‘A’ and ‘B’ biotypes of Bemisia tabaci. Insect Science and Its Application, 14, 127–138.
De Barro, P. J., Liu, S.-S., Boykin, L. M., & Dinsdale, A. B. (2011). Bemisia tabaci: a statement of species status. Annual Review of Entomology, 56, 1–19.
De la Rua, P., Simon, B., Cifuentes, D., Martinez-Mora, C., & Cenis, J. L. (2006). New insights into the mitochondrial phylogeny of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) in the Mediterranean Basin. Journal of Zoological Systematics and Evolutionary Research, 44, 25–33.
Dinsdale, A., Cook, L., Riginos, C., Buckley, Y. M., & De Barro, P. (2010). Refined global analysis of Bemisia tabaci (Hemiptera: Sternorrhyncha: Aleyrodoidea: Aleyrodidae) mitochondrial cytochrome oxidase I to identify species level genetic boundaries. Annals of the Entomological Society of America, 103, 196–208.
Elfekih, S., Tay, W. T., Gordon, K., Courta, L. N., & De Barro, P. J. (2018). Standardized molecular diagnostic tool for the identification of cryptic species within the Bemisia tabaci complex. Pest Management Science, 74, 170–173.
Fidan, H., Karacaoglu, M., Koc, G., & Caglar, B. K. (2019). Tomato yellow leaf curl virus (TYLCV) strains and epidemiological role of Bemisia tabaci (Hemiptera: Aleyrodidae) biotypes on tomato agroecology in Turkey. Applied Ecology and Environmental Research, 17, 9131–9144.
Gerling, D. (1996). Status of Bemisia tabaci in the Mediterrenean countries: Opportunities for biological control. Biological Control, 6, 11–22.
Gottlieb, Y., Zchori-Fein, E., Mozes-Daube, N., Kontsedalov, S., Skaljac, M., Brumin, M., Sobol, I., Czosnek, H., Vavre, F., Fleury, F., & Ghanim, M. (2010). The transmission efficiency of tomato yellow leaf curl virus by the whitefly Bemisia tabaci is correlated with the presence of a specific symbiotic bacterium species. Journal of Virology, 84, 9310–9317.
Gueguen, G., Vavre, F., Gnankine, O., Peterschmitt, M., Charif, D., Chiel, E., et al. (2010). Endosymbiont meta communities, mtDNA diversity and the evolution of the Bemisia tabaci (Hemiptera: Aleyrodidae) species complex. Molecular Ecology, 19, 4365–4376.
Hu, J., De Barro, P., Zhao, H., Wang, J., Nardi, F., & Liu, S. S. (2011). An extensive field survey combined with a phylogenetic analysis reveals rapid and widespread invasion of two alien whiteflies in China. PLoS One, 6, 1–9.
Karut, K., & Tok, B. (2014). Secondary endosymbionts of Turkish Bemisia tabaci (Gennadius) populations. Phytoparasitica, 42, 413–419.
Karut, K., Kazak, C., Doker, I., & Ulusoy, M. R. (2011). Pest status and prevalence of tomato moth Tuta absoluta (Meyrick 1917) (Lepidoptera: Gelechiidae) in tomato growing greenhouses of Mersin. Turkish Journal of Entomology, 35, 339–347.
Karut, K., Kaydan, M. B., Castle, S. J., Kazak, C., & Ulusoy, M. R. (2014). Study on species composition of Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae) on cotton in Cukurova plain, Turkey. Turkish Journal of Entomology, 38, 43–50.
Karut, K., Kaydan, M. B., Tok, B., Doker, I., & Kazak, C. (2015). A new record for Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) species complex of Turkey. Journal of Applied Entomology, 139, 158–160.
Karut, K., Karaca, M. M., Döker, I., & Kazak, C. (2017). Analysis of species, subgroups, and endosymbionts of Bemisia tabaci (Hemiptera: Aleyrodidae) from southwestern cotton fields in Turkey. Environmental Entomology, 46, 1035–1040.
Kazak, C., Karut, K., Yildiz, S., & Sekeroglu, E. (1998). Biological, control of Thrips tabaci Lindeman (Thysanoptera: Thripidae) by Amblyseius cucumeris (Oudemans) (Acarina: Phytoseiidae) on greenhouse cucumber in Adana, Turkey. Acta Hortıculturae, 492, 169–174.
Kliot, A., & Ghanim, M. (2013). The role of bacterial chaperones in the circulative transmission of plant viruses by insect vectors. Viruses, 5, 1516–1535.
Köklü, G., Rojas, A., & Kvarnheden, A. (2006). Molecular identification and the complete nucleotide sequence of a tomato yellow leaf curl virus isolate from Turkey. Journal of Plant Pathology, 88, 61–66.
Kontsedalov, S., Abu-Moch, F., Lebedev, G., Czosnek, H., Horowitz, A. R., & Ghanim, M. (2012). Bemisia tabaci biotype dynamics and resistance to insecticides in Israel during the years 2008-2010. Journal of Integrative Agriculture, 11, 312–320.
Laarif, A., Saleh, D., Clouet, C., & Gauthier, N. (2015). Regional co-occurrence between distinct Bemisia tabaci species in Tunisia with new insights into the role of host plants. Phytoparasitica, 43, 135–150.
Lee, W., Lee, S. M., Kim, C. S., Choi, H. S., Akimoto, S. I., Lee, K. Y., et al. (2014). Three species of the Bemisia tabaci (Hemiptera: Aleyrodidae) complex in the Republic of Korea; detection by an extensive field survey combined with a phylogenetic analysis. Florida Entomologist, 97, 155–161.
Legg, J. P., Shirima, R., Tajebe, L. S., Guastella, D., Boniface, S., Jeremiah, S., Nsami, E., Chikoti, P., & Rapisarda, C. (2014). Biology and management of Bemisia whitefly vectors of cassava virus pandemics in Africa. Pest Management Science, 70, 1446–1453.
McKenzie, C. L., Anderson, P. K., & Villarreal, N. (2004). An extensive survey of Bemisia tabaci (Homoptera: Aleyrodidae) in agricultural ecosystems in Florida. Florida Entomologist, 7, 403–407.
McKenzie, C. L., Bethke, J. A., Byrne, F. J., Chamberlin, J. R., Dennehy, T. J., Dickey, A. M., et al. (2012). Distribution of Bemisia tabaci (Hemiptera: Aleyrodidae) B biotypes in North America after the Q invasion. Journal of Economic Entomology, 105, 753–766.
Pascual, S., & Callejas, C. (2004). Intra- and interspecific competition between biotypes B and Q of Bemisia tabaci (Hemiptera: Aleyrodidae) from Spain. Bulletin of Entomological Research, 94, 369–375.
Saleh, D., Laarif, A., Clouet, C., & Gauthier, N. (2012). Spatial and host-plant partitioning between coexisting Bemisia tabaci cryptic species in Tunisia. Population Ecology, 54, 261–274.
Stansly, P. A., & Naranjo, S. E. (2010). Introduction. In I. P. A. Stansly & S. E. Naranjo (Eds.), Bemisia, bionomics and management of a global pest (pp. XV–XVIII). New York: Springer+Business.
Tajebe, L. S., Boni, S. B., Guastella, D., Cavalieri, V., Lund, O. S., Rugumamu, C. P., Rapisarda, C., & Legg, J. P. (2015). Abundance, diversity and geographic distribution of cassava mosaic disease pandemic-associated Bemisia tabaci in Tanzania. Journal of Applied Entomology, 139, 627–637.
Tüzel, Y., & Öztekin, G. B. (2015). Protected cultivation in Turkey. Chronica Horticulture, 55, 21–26.
Ulusoy, M. R., Brown, J. K., & Bayhan, E. (2002). The ‘B’ biotype of Bemisia tabaci now established in Turkey. European Studies Network on European Whiteflies Newsletter, No. 13.
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This research was part of the MSc thesis of İlksen Topçu which was supported by the Çukurova University Research Foundation, under Grant FYL-2016-6665.
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Topçu, İ., Karaca, M.M. & Karut, K. Dominance of Bemisia tabaci MEAM1 species over MED (Hemiptera: Aleyrodidae) in greenhouse vegetables in Mersin, Turkey. Phytoparasitica 48, 159–166 (2020). https://doi.org/10.1007/s12600-020-00794-8
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DOI: https://doi.org/10.1007/s12600-020-00794-8