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Effects of culture medium composition and PEG on hyperhydricity in Dendrobium officinale

  • Abiotic Stress Responses
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Abstract

Hyperhydricity is a physiological disorder during plant tissue culture that seriously affects regeneration and micropropagation. In this study, Dendrobium officinale plantlets were cultured on solid Murashige and Skoog (MS) medium supplemented with plant growth regulators and various concentrations of sucrose, agar, and polyethylene glycol (PEG)-6000 to explore the effect of osmotic stress on hyperhydricity. The results show that low concentrations of sucrose or agar, as well as PEG-6000 at various concentrations, significantly increase the hyperhydric rate of D. officinale, whereas high concentrations of sucrose or agar did not. Furthermore, high concentrations of PEG-6000 significantly increase total water content, free-water content, relative electrical conductivity, and peroxidase (POD) activity of D. officinale plantlets, whereas they significantly decrease bound-water content, proline content, soluble protein content, soluble sugar content, and superoxide dismutase (SOD) activity. These results indicate that PEG-6000 disrupts the antioxidant system and water metabolism in D. officinale plantlets, as well as increases cell membrane permeability, which might be the key factors for the occurrence of hyperhydricity in this species.

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Acknowledgments

The basic research project of Luoding Cinnamon Industry Development (2018-082), Scientific Research Project of the Traditional Chinese Medicine Bureau of Guangdong Province (20202104), and the seedling fund of the School of Traditional Chinese Medicine (YM1901) supported this study.

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Authors and Affiliations

  1. School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Hongyang Gao, Danyun Xu, Huiju Zhang, Xuanxuan Cheng & Quan Yang

  2. Guangdong Provincial Research Center on Good Agricultural Practice & Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials, Guangzhou, 510006, China

    Quan Yang

  3. Comprehensive Experimental Station of Guangzhou, Chinese Material Medica, China Agriculture Research System (CARS-21-16), Guangzhou, 510006, China

    Quan Yang

  4. Key Laboratory of State Administration of Traditional Chinese Medicine for Production & Development of Cantonese Medicinal Materials, Guangzhou, 510006, China

    Quan Yang

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  1. Hongyang Gao
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  2. Danyun Xu
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  3. Huiju Zhang
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  4. Xuanxuan Cheng
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  5. Quan Yang
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Correspondence to Xuanxuan Cheng or Quan Yang.

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Editor: Yong Eui Choi

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Gao, H., Xu, D., Zhang, H. et al. Effects of culture medium composition and PEG on hyperhydricity in Dendrobium officinale. In Vitro Cell.Dev.Biol.-Plant 56, 143–149 (2020). https://doi.org/10.1007/s11627-020-10075-y

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  • DOI: https://doi.org/10.1007/s11627-020-10075-y

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