Abstract
One of the strategies for genetic improvement of Oryza sativa L. (rice) is the use of radiation-induced mutagenesis to search for materials suitable for production in marginal conditions, such as drought and salinity. In this present study, indica rice ‘CR-5272’ embryogenic calluses and seeds were exposed to gamma radiation. Survival and growth of plants derived from embryogenic calluses and seeds were evaluated in vitro in the presence of selection factors for salinity and drought (NaCl and sorbitol). Plants regenerated from irradiated calluses and seeds were screened for tolerance to these conditions and promising lines were identified. Culture of plants regenerated from irradiated calluses (M1) allowed the selection of 60, 58 and 15 promising lines with tolerance to salinity, drought, and both factors, respectively. Six promising salt-tolerant lines and four drought-tolerant lines were selected from plants grown from irradiated seeds (M2). A hormetic response to irradiation was observed in seeds; seeds irradiated at 50 Gy showed increased callus induction and callus weight. This study allowed advancements in the improvement of rice ‘CR-5272’ through the production of promising lines that are being evaluated in field studies.
Similar content being viewed by others
References
Ahloowalia BS, Maluszynski M (2001) Induced mutations—a new paradigm in plant breeding. Euphytica 118:167–173. https://doi.org/10.1023/A:100416232
Al-Enezi NA, Al-Khayri JM (2012) Effect of X-irradiation on proline accumulation, growth, and water content of date palm (Phoenix dactylifera L.) seedlings. J Biol Sci 12:146–153. https://doi.org/10.3923/jbs.2012.146.153
Amini S, Ghadiri H, Chen C, Marschner P (2016) Salt-affected soils, reclamation, carbon dynamics, and biochar: a review. J Soils Sediments 16:939–953. https://doi.org/10.1007/s11368-015-1293-1
Bado S, Forster BP, Nielen S, Ghanim A, Lagoda PJ, Till BJ, Laimer M (2015) Plant mutation breeding: current progress and future assessment. In: Goldman IL, Michler CH, Ortiz R (eds) Plant breeding reviews. Willey-Blackwell, Hoboken, NJ, pp 23–88 ISSN: 0730-2207
Bai C, Rivera SM, Medina V, Alves R, Vilaprinyo E, Sorribas A, Zhu C (2014) An in vitro system for the rapid functional characterization of genes involved in carotenoid biosynthesis and accumulation. Plant J 77:464–475. https://doi.org/10.1111/tpj.12384
Baldwin J, Grantham V (2015) Radiation hormesis: historical and current perspectives. J Nucl Med 43:242–246. https://doi.org/10.2967/jnmt.115.166074
Bündig C, Vu TH, Meise P, Seddig S, Schum A, Winkelmann T (2017) Variability in osmotic stress tolerance of starch potato genotypes (Solanum tuberosum L.) as revealed by an in vitro screening: role of proline, osmotic adjustment and drought response in pot trials. J Agron Crop Sci 203:206–218. https://doi.org/10.1111/jac.12186
Chemical Laboratory ARVI S. A (2015) Supresor Microbiológico S.B. ARVI: Inhibidor/controlador de contaminación para cultivos in vitro, Ficha técnica, Online document http://www.arvicr.com/productos/fichas-tecnicas/solucion-biocidaft.pdf. Accessed on January 15, 2019
Clarke D, Lázár AN, Saleh AFM, Jahiruddin M (2018) Prospects for agriculture under climate change and soil salinisation. In: Nicholls RJ, Hutton CW, Adger WN, Hanson SE, Rahman M, Salehin M (eds) Ecosystem services for well-being in deltas. Palgrave Macmillan, Cham, pp 447–467
Compton ME, Koch JM (2001) Influence of plant preservative mixture (PPM) TM on adventitious organogenesis in melon, petunia, and tobacco. In Vitro Cell Dev Biol–Plant 37:259–261. https://doi.org/10.1079/IVP2000144
Food and Agriculture Organization of the United Nations/International Atomic Energy Agency (FAO/IAEA) (2018) Mutant Variety Database. http://mvdiaeaorg/ Cited 5 October 2018
Hase Y, Satoh K, Kitamura S, Oono Y (2018) Physiological status of plant tissue affects the frequency and types of mutations induced by carbon-ion irradiation in Arabidopsis. Sci Rep 8:1394–1404. https://doi.org/10.1038/s41598-018-19278-1
Hedayati P, Monfard HH, Isa NM, Hwang DJ, Zain CRCM, Uddin MI, Zainal Z (2015) Construction and analysis of an Oryza sativa (cv. MR219) salinity-related cDNA library. Acta Physiol Plant 37:91. https://doi.org/10.1007/s11738-015-1837-4
International Rice Research Institute (2018) World rice statistics online query facility. http://ricestatirriorg:8080/wrsv3/entrypointhtm Cited 9 October 2018
Jiménez VM, Castillo J, Tavares E, Guevara E, Montiel M (2006) In vitro propagation of the neotropical giant bamboo, Guadua angustifolia Kunth, through axillary shoot proliferation. Plant Cell Tissue Organ Cult 86:389–395. https://doi.org/10.1007/s11240-006-9120-4
Khanam B, Chandra R (2017) Optimization of surface sterilization process of selected dye-yielding plants for isolation of bacterial endophytes. In: Mukhopadhyay K, Sachan A, Kumar M (eds) Applications of biotechnology for sustainable development. Springer, Singapore, pp 45–50. https://doi.org/10.1007/978-981-10-5538-6_7
Khush GS (2013) Strategies for increasing the yield potential of cereals: case of rice as an example. Plant Breed 132:433–436. https://doi.org/10.1111/pbr.1991
Maluszynski M, Szarejko I, Bhatia CR, Nichterlein K, Lagoda PJ (2009) Methodologies for generating variability Part 4, Mutation techniques. In: Ceccarelli S, Guimaraes EP, Weltzien E (eds) Plant breeders and farmer participation. FAO, Rome, Italy, pp 159–194 ISBN 978–92–5-106382-8
Marssaro AL, Morais-Lino LS, Cruz JL, Ledo CA, Santos-Serejo JA (2017) Simulation of in vitro water deficit for selecting drought-tolerant banana genotypes. Pesq Agropec Bras 52:1301–1304. https://doi.org/10.1590/s0100-204x2017001200021
Masoabi M, Lloyd J, Kossmann J, van der Vyver C (2018) Ethyl methanesulfonate mutagenesis and in vitro polyethylene glycol selection for drought tolerance in sugarcane (Saccharum spp.). Sugar Tech 20:50–59. https://doi.org/10.1007/s12355-017-0524-8
Mba C (2013) Induced mutation unleashes the potentials of plant genetic resources for food and agriculture. Agronomy 3:200–231. https://doi.org/10.3390/agronomy3010200
Minitab 17 (2010) Statistical Software by Minitab Inc. Retrieved from http://www.minitab.com/es-mx/products/minitab/ on December 13, 2018
Miyazaki J, Tan BH, Errington SG (2010) Eradication of endophytic bacteria via treatment for axillary buds of Petunia hybrida using Plant Preservative Mixture (PPM™). Plant Cell Tissue Organ Cult 102:365–372. https://doi.org/10.1007/s11240-010-9741-5
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Orlikowska T, Nowak K, Reed B (2017) Bacteria in the plant tissue culture environment. Plant Cell Tissue Organ Cult 128:487–508. https://doi.org/10.1007/s11240-016-1144-9
Prasanna S, Jain SM (2017) Mutant resources and mutagenomics in crop plants. Emir J Food Agric 29:651–657. https://doi.org/10.9755/ejfa.2017.v29.i9.86
Razavizadeh R, Adabavazeh F (2017) Effects of sorbitol on essential oil of Carum copticum L. under in vitro culture. Rom Biotechnol Lett 22:12281–12289 https://www.rombio.eu/vol22nr1/16_Raazavideh.pdf. Accessed 21 Nov 2018
Reddy IN, Kim BK, Yoon IS, Kim KH, Kwon TR (2017) Salt tolerance in rice: focus on mechanisms and approaches. Rice Sci 24:123–144. https://doi.org/10.1016/j.rsci.2016.09.004
Robertson AD, Zhang Y, Sherrod LA, Rosenzweig ST, Ma L, Ahuja L, Schipanski ME (2018) Climate change impacts on yields and soil carbon in row crop dryland agriculture. J Environ Qual 47:684–694. https://doi.org/10.2134/jeq2017.08.0309
Schaart JG, van de Wiel CC, Lotz LA, Smulders MJ (2016) Opportunities for products of new plant breeding techniques. Trends Plant Sci 21:438–449. https://doi.org/10.1016/j.tplants.2015.11.006
Schiocchet MA, Noldin JA, Raimondi JV, Neto AT, Marschalek R, Wickert E, Eberhardt DS (2014) SCS118 Marques—new rice cultivar obtained through induced mutation. Crop Breed Appl Biotechnol 14:68–70. https://doi.org/10.1590/S1984-70332014000100012
Silvetti T, Merlini L, Brasca M, Galante YM (2018) Aerogel from chemo-enzymatically oxidized fenugreek gum: an innovative delivery system of isothiazolinones biocides. Appl Microbiol Biotechnol 102:2683–2692. https://doi.org/10.1007/s00253-018-8804-0
Smith RH (2013) Plant tissue culture: techniques and experiments, vol 3. Academic Press. San Diego, California, USA, pp 45–52. https://doi.org/10.1016/B978-0-12-415920-4.00004-9
Sudhakar D, Bong BB, Tinjuangjun P, Maqbool SB, Valdez M, Jefferson R, Christou P (1998) An efficient rice transformation system utilizing mature seed-derived explants and a portable, inexpensive particle bombardment device. Transgenic Res 7:289–294. https://doi.org/10.1023/A:1008870012568
Tai TH (2007) Induced mutations in rice (Oryza sativa L.). Isr J Plant Sci 55:137–145. https://doi.org/10.1560/IJPS.55.2.137
Yan W, Hu B, Zhang Q, Jia L, Jackson A, Pan X, Huang B, Yan Z, Deren C (2012) Short and erect rice (ser) mutant from ‘Khao Dawk Mali 105’ improves plant architecture. Plant Breed 131:282–285. https://doi.org/10.1111/j.1439-0523.2011.01943.x
Funding
The authors gratefully recognize the financial support of the Consejo Nacional de Rectores (CONARE) of Costa Rica (VI-252-2015) and the Vice Presidencies for Research and Extension of the Instituto Tecnologico de Costa Rica and the Universidad de Costa Rica.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Editor: Wagner Campos Otoni
Rights and permissions
About this article
Cite this article
Abdelnour-Esquivel, A., Perez, J., Rojas, M. et al. Use of gamma radiation to induce mutations in rice (Oryza sativa L.) and the selection of lines with tolerance to salinity and drought. In Vitro Cell.Dev.Biol.-Plant 56, 88–97 (2020). https://doi.org/10.1007/s11627-019-10015-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11627-019-10015-5