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Utilization of Microbial Oil from Poplar Wood Hemicellulose Prehydrolysate for the Production of Polyol Using Chemo-enzymatic Epoxidation

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  • Bioprocess Engineering
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Abstract

The aim of this work was to demonstrate the production of polyol from microbial oil using wood-based hemicellulose stream through a greener enzymatic process. Yeast biomass concentration of 16.54 ± 0.65 g/L and lipid concentration of 6.97 ± 0.58 g/L were obtained by batch fermentation of hemicellulose prehydrolysate of poplar wood using an oleaginous yeast Cryptococcus curvatus. The produced microbial oil was successfully converted to epoxidized oil catalyzed by lipase with 84.55 ± 1.80% conversion. The epoxidation followed by ring opening reaction to produce polyol with hydroxyl value of 299.53 ± 1.24 mg KOH/g. This showed the possible use of the hemicellulose stream of lignocellulosic biomass to microbial oil and its subsequent conversion to polyol, a precursor to polyurethane and a number of value-added products. This bio-based polyol could be used to substitute the conventional polyols.

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Abbreviations

A i :

Atomic weight of iodine

A 0 :

Atomic weight of oxygen

AIER:

Acid ion exchange resin

CALB:

Candida antarctica lipase

HMF:

Hydroxy methyl furfural

IV:

Iodine value

MO:

Microbial oil

OO exp :

Experimental oxirane value

OO th :

Theorical oxirane value

PHL:

Prehydrolysate liquor

TGA:

Triacylglycerides

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Acknowledgments

We would like to thank Greenfield Global Inc., Chatham, Canada, for the supply of the hemicellulose substrate.

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Authors and Affiliations

  1. Department of Biotechnology, Lakehead University, Thunder Bay, Ontario, Canada

    Mahdieh Samavi & Sudip Rakshit

  2. Department of Chemical Engineering, Lakehead University, Thunder Bay, Ontario, Canada

    Sudip Rakshit

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  1. Mahdieh Samavi
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Correspondence to Sudip Rakshit.

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Samavi, M., Rakshit, S. Utilization of Microbial Oil from Poplar Wood Hemicellulose Prehydrolysate for the Production of Polyol Using Chemo-enzymatic Epoxidation. Biotechnol Bioproc E 25, 327–335 (2020). https://doi.org/10.1007/s12257-019-0416-8

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