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Effective Elimination of Charge-associated Toxicity of Low Generation Polyamidoamine Dendrimer Eases Drug Delivery of Oxaliplatin

  • Research Paper
  • Nanobiotechnology
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Abstract

Polyamidoamine (PAMAM) dendrimer is emerging as an effective nanocarrier for delivering anticancer drugs. Still, unmodified PAMAM dendrimer is hardly used in vivo because of unsatisfied drug release, high tendency of interfering with cellular membranes, and rapid clearance by reticuloendothelial system. In this study, low generation polyamidoamine (PAMAM) dendrimer G3.0 is developed and surface modified with methoxypolyethylene glycol (PAMAM G3.0-mPEG) to overcome its limitations. Specifically, PAMAM G3.0 conjugated with mPEG at different ratios are investigated to effectively eliminate its charge-associated toxicity, in which PAMAM G3.0-mPEG- 8 is chosen for oxaliplatin (OX) loading. Results reveal that OX-loaded PAMAM G3.0-mPEG-8 has desirable size, good entrapment efficiency, and sustained release with minimum drug leakage. In addition, Resazurin assay indicates that the toxicity of loaded OX is reduced as compared to free drug but still maintain substantially anticancer activity on HeLa cells, suggesting the potential application of PAMAM G3.0-mPEG-8 for OX delivery in cancer therapy.

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Authors and Affiliations

  1. Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City, 700000, Vietnam

    Vo Minh Hoang Do, Diem-Huong Nguyen Tran & Dai Hai Nguyen

  2. Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, 100000, Vietnam

    Vo Minh Hoang Do & Dai Hai Nguyen

  3. Nguyen Tat Thanh University, Ho Chi Minh City, 700000, Vietnam

    Long Giang Bach

  4. Faculty of Pharmacy, Lac Hong University, Buu Long Ward, Bien Hoa City, Dong Nai Province, 810000, Vietnam

    Van Du Cao, Thi Nhu Quynh Nguyen, Duc Thuan Hoang & Van Cuong Ngo

  5. Biomaterials and Nanotechnology Research Group, Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, 700000, Vietnam

    Thai Thanh Hoang Thi

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Effective Elimination of Charge-associated Toxicity of Low Generation Polyamidoamine Dendrimer Eases Drug Delivery of Oxaliplatin

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Do, V.M.H., Bach, L.G., Tran, DH.N. et al. Effective Elimination of Charge-associated Toxicity of Low Generation Polyamidoamine Dendrimer Eases Drug Delivery of Oxaliplatin. Biotechnol Bioproc E 25, 224–234 (2020). https://doi.org/10.1007/s12257-019-0047-0

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