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Development of an eco-friendly antibacterial textile: lysozyme immobilization on wool fabric

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Abstract

Lysozyme, a type of natural enzyme, has been widely used for bacteriostatic functionalization of various materials due to its efficient and selective antibacterial properties. Herein, we report the preparation and characterization of an eco-friendly antibacterial textile based on the immobilization of lysozyme from chicken egg white onto wool fibers. Tris(hydroxymethyl)phosphine (THP) was employed as the cross-linker for the immobilization of lysozyme on the surface of wool fiber. The mechanism of THP cross-linking was investigated via phosphorus test, energy-dispersive spectroscopy (EDX) and Fourier transform infrared spectroscopy (FT-IR). The surface staining, optimization of immobilization parameters, morphology, antibacterial properties, and durability of wool fibers with immobilized lysozyme were also assessed. The results show that hydroxymethyl groups of THP reacted with amino groups of wool fiber and lysozyme through Mannich reaction, which successfully immobilized lysozyme on the wool fiber. The wool fibers incorporated with lysozyme had better antibacterial properties and durability compared with the untreated wool fabric. This facile immobilization approach of lysozyme provides an effective strategy for environmentally benign modification and functionalization of keratin and keratin-containing materials.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (51673087), the National Key R&D Program of China (2017YFB0309200) and the Fundamental Research Funds for the Central Universities (JUSRP51717A).

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Authors and Affiliations

  1. Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan, University, Wuxi, 214122, Jiangsu, China

    Wenli Yang, Nan Zhang, Qiang Wang, Ping Wang & Yuanyuan Yu

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  1. Wenli Yang
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  2. Nan Zhang
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  3. Qiang Wang
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  4. Ping Wang
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  5. Yuanyuan Yu
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Correspondence to Qiang Wang.

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Yang, W., Zhang, N., Wang, Q. et al. Development of an eco-friendly antibacterial textile: lysozyme immobilization on wool fabric. Bioprocess Biosyst Eng 43, 1639–1648 (2020). https://doi.org/10.1007/s00449-020-02356-y

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  • DOI: https://doi.org/10.1007/s00449-020-02356-y

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