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Identifying the compounds that can distinguish between Saposhnikovia root and its substitute, Peucedanum ledebourielloides root, using LC-HR/MS metabolomics

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Abstract

Previously, we established a 1H NMR metabolomics method using reversed-phase solid-phase extraction column (RP-SPEC), and succeeded in distinguishing wild from cultivated samples of Saposhnikoviae radix (SR), and between SR and its substitute, Peucedanum ledebourielloides root (PR). Herein, we performed LC-HR/MS metabolomics using fractions obtained via RP-SPEC to identify characteristic components of SR and PR. One and three characteristic components were respectively found for SR and PR; these components were isolated with their m/z values and retention times as a guide. The characteristic component of SR was identified as 4′-O-β-d-glucosyl-5-O-methylvisamminol (1), an indicator component used to identify SR in the Japanese Pharmacopoeia. In contrast, the characteristic components of PR were identified as xanthalin (2), 4′-O-β-d-apiosyl (1 → 6)-β-d-glucosyl-5-O-methylvisamminol (3), and 3′-O-β-d-apiosyl (1 → 6)-β-d-glucosylhamaudol (4) based on spectroscopic data such as 1D- and 2D-NMR, MS, and specific optical rotation. Among them, 4 is a novel compound. For the correlation between the NMR metabolomics results in the present and our previous report, only 1 and 2 were found to correlate with the chemical shifts, and the other compounds had no correlation. As the chemical shifts for compounds 1, 3, and 4 were similar to each other, especially for the aglycone moiety, they could not be distinguished because of the sensitivity and resolution of 1H NMR. Accordingly, combining NMR and LC/MS metabolomics with their different advantages is considered useful for metabolomics of natural products. The series of methods used in our reports could aid in quality evaluations of natural products and surveying of marker components.

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Acknowledgements

This study was supported by a research grant from the Japan Agency for Medical Research and Development (the grant number: 17ak0101074j2001, 15ak0101017h0003).

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Authors and Affiliations

  1. Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, 210-9501, Japan

    Taichi Yoshitomi, Nahoko Uchiyama, Takashi Tsujimoto, Takashi Hakamatsuka & Takuro Maruyama

  2. Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo, 142-8501, Japan

    Daigo Wakana

  3. Research Center for medicinal Plant Resources, National Institutes of Biomedical Innovation, Health and Nutrition, 1-2 Hachimandai, Tsukuba, Ibaraki, 305-0843, Japan

    Noriaki Kawano, Hiroyuki Fuchino & Nobuo Kawahara

  4. Nippon Funmatsu Yakuhin Co., Ltd, 71-3 Izumicho, Yao-shi, Osaka, 581-0813, Japan

    Tsuguo Yokokura

  5. Tochimoto Tenkaido Co., Ltd, Oniya Kaibara-Cho, Tamba, Hyogo, 669-3315, Japan

    Yutaka Yamamoto

  6. Department of Medicinal Resources Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan

    Katsuko Komatsu

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  1. Taichi Yoshitomi
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  2. Daigo Wakana
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  12. Takuro Maruyama
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Correspondence to Takuro Maruyama.

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Yoshitomi, T., Wakana, D., Uchiyama, N. et al. Identifying the compounds that can distinguish between Saposhnikovia root and its substitute, Peucedanum ledebourielloides root, using LC-HR/MS metabolomics. J Nat Med 74, 550–560 (2020). https://doi.org/10.1007/s11418-020-01409-6

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  • DOI: https://doi.org/10.1007/s11418-020-01409-6

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