Skip to main content

Advertisement

SpringerLink
Log in

The seasonal changes of core bacterial community decide sewage purification in sub-plateau municipal sewage treatment plants

  • Research Paper
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

The decline of sewage purification efficiency in winter is a frequent problem in sub-plateau municipal sewage treatment plants (MSTPs). Understanding the links between activated sludge (AS) bacterial community and sewage purification is crucial for exploring the cause of this problem. In this study, Illumina high-throughput sequencing technology was applied to investigate the seasonal changes of AS bacterial community in sub-plateau MSTPs. The sequencing result indicates that the bacterial community OTU number, diversity, and relative abundance in winter are significantly lower than that in summer samples. The discriminant linear effect size analysis (LEfSe) reveals that Proteobacteria and Chloroflexi members were enriched in summer AS, while Actinobacteria and Firmicutes were enriched in winter AS. The results indicate that different core bacterial community assembly was developed in summer and winter, respectively. The changes in bacterial community may be the reasons for the lower sewage purification efficiency in winter. Furthermore, redundancy analysis (RDA) shows that temperature and dissolved oxygen (DO) are the principal factors that drive the seasonal changes in the core bacterial community diversity, richness and structure in sub-plateau MSTPs. Thus, the sub-plateau AS selects for a unique community assembly pattern and shapes the particular AS ecosystem. These results expand previous understanding and provide insight into the relationship between bacterial community and performance of sub-plateau MSTPs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Alejandro G-M, Maija S, Barbara M-P, Alejandro R-S, Anna M, Riku V (2018) Microbial ecology of full-scale wastewater treatment systems in the Polar Arctic Circle: Archaea Bacteria and Fungi. Sci Rep-UK 8:1–11

    Article  Google Scholar 

  2. Andreasen K, Nielsen PH (2000) Growth of Microthrix parvicella in nutrient removal activated sludge plants: studies of in situ physiology. Water Res 34:1559–1569

    Article  CAS  Google Scholar 

  3. Caporaso JG, Lauber CL, Walters WA, Berg-Lyons D, Huntley J, Fierer N, Owens SM, Betley J, Fr aser L, Bauer M, Gormley N, Gilbert JA, Smith G, Knight R (2012) Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms. ISME J 6:1621–1624

    Article  CAS  Google Scholar 

  4. Chen H, Chang S (2017) Impact of temperatures on microbial community structures of sewage sludge biological hydrolysis. Bioresource Technol 245(Pt A):502–510

    Article  CAS  Google Scholar 

  5. Chen Y, Lan S, Wang L, Dong S, Zhou H, Tan L, Li X (2017) A review: Driving factors and regulation strategies of microbial community structure and dynamics in wastewater treatment systems. Chemosphere 174:173–182

    Article  CAS  Google Scholar 

  6. Chu Z, Wang K, Li X, Zhu M, Yang L, Zhang J (2015) Microbial characterization of aggregates within a one-stage nitritation–anammox system using high-throughput amplicon sequencing. Chem Eng J 262:41–48

    Article  CAS  Google Scholar 

  7. Fan X, Gao J, Pan K, Li D, Dai H, Li X (2018) Functional genera, potential pathogens and predicted antibiotic resistance genes in 16 full-scale wastewater treatment plants treating different types of wastewater. Bioresource Technol 268:97–106

    Article  CAS  Google Scholar 

  8. Fan X, Gao J, Pan K, Li D, Dai H (2017) Temporal dynamics of bacterial communities and predicted nitrogen metabolism genes in a full-scale wastewater treatment plant. RSC Adv 7(89):56317–56327

    Article  CAS  Google Scholar 

  9. Fang D, Zhao G, Xu X (2018) Microbial community structures and functions of wastewater treatment systems in plateau and cold regions. Bioresource Technol 249:684–693

    Article  CAS  Google Scholar 

  10. Gao J, Liu G, Li H, Xu L, Du L, Yang B (2016) Predictive functional profiling using marker gene sequences and community diversity analyses of microbes in full-scale anaerobic sludge digesters. Bioprocess Biosyst Eng 39(7):1115–1127

    Article  CAS  Google Scholar 

  11. Guo J, Peng Y, Ni B, Han X, Fan L, Yuan Z (2015) Dissecting microbial community structure and methane-producing pathways of a full-scale anaerobic reactor digesting activated sludge from wastewater treatment by metagenomic sequencing. Microbe Cell Fact 14(1):1–11

    Article  Google Scholar 

  12. Ibarbalz FM, Figuerola ELM, Erijman L (2013) Industrial activated sludge exhibits unique bacterial community composition at high taxonomic ranks. Water Res 47:3854–3864

    Article  CAS  Google Scholar 

  13. Jin LY, Zhang GM, Tian HF (2014) Current state of sewage treatment in China. Water Res 66:85–98

    Article  CAS  Google Scholar 

  14. Kang XH, Wu XK, Wang H, Zeng XY, Leng Y, Li SW (2018) Illumina sequencing reveals bacterial community shift and its role in a full-scale A2O sewage treatment process at low temperatures. Desalin Water Treat 109:193–204

    Article  CAS  Google Scholar 

  15. Kurumi H, Masami M, Daisuke I, Michihiko I (2014) Bacterial community dynamics in a full-scale municipal wastewater treatment plant employing a conventional activated sludge process. J Biosci Bioeng 118(1):64–71

    Article  Google Scholar 

  16. Lee SH, Kang HJ, Park HD (2015) Influence of influent wastewater communities on temporal variation of activated sludge communities. Water Res 73:132–144

    Article  CAS  Google Scholar 

  17. Ma S, Ding L, Huang H, Geng J, Xu K, Zhang Y, Ren H (2016) Effects of DO levels on the surface force, cell membrane properties and microbial community dynamics of activated sludge. Bioresour Technol 214:645–652

    Article  CAS  Google Scholar 

  18. Muszynski A, Tabernacka A, Milobedzka A (2015) Long-term dynamics of the microbial community in a full-scale wastewater treatment plant. Int Biodeterior Biodegrad 100:44–51

    Article  CAS  Google Scholar 

  19. Niu L, Li Y, Wang P, Zhang W, Wang C, Cai W, Wang L (2016) Altitude-scale variation in nitrogen-removal bacterial communities from municipal wastewater treatment plants distributed along a 3600-m latitudinal gradient in China. Sci Total Environ 559:38–44

    Article  CAS  Google Scholar 

  20. Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett W.S, Huttenhower C (2011) Metagenomic biomarker discovery and explanation. Genome Biol 12(6)

  21. Seib MD, Berg KJ, Zitomer DH (2016) Influent wastewater microbiota and temperature influence anaerobic membrane bioreactor microbial community. Bioresource Technol 216:446–452

    Article  CAS  Google Scholar 

  22. Sims A, Gajaraj S, Hu ZQ (2012) Seasonal population changes of ammonia-oxidizing organisms and their relationship to water quality in a constructed wetland. Ecol Eng 40:100–107

    Article  Google Scholar 

  23. Stadler LB, Love NG (2016) Impact of microbial physiology and microbial community structure on pharmaceutical fate driven by dissolved oxygen concentration in nitrifying bioreactors. Water Res 104:189–199

    Article  CAS  Google Scholar 

  24. Wang P, Yu Z, Zhao J, Zhang H (2016) Seasonal changes in bacterial communities cause foaming in a wastewater treatment plant. Microb Ecol 71(3):660–671

    Article  CAS  Google Scholar 

  25. Wells GF, Wu CH, Piceno YM, Eggleston B, Brodie EL, DeSantis TZ, Andersen GL, Hazen TC, Francis CA, Criddle CS (2014) Microbial biogeography across a full-scale wastewater treatment plant transect: evidence for immigration between coupled processes. Appl Microbiol Biotechnol 98(10):4723–4736

    Article  CAS  Google Scholar 

  26. Xia Y, Wen X, Zhang B, Yang Y (2018) Diversity and assembly patterns of activated sludge microbial communities: a review. Biotechnol Adv 36:1038–1047

    Article  Google Scholar 

  27. Yoon DN, Park SJ, Kim SJ (2010) Isolation, characterization, and abundance of filamentous members of Caldilineae in activated sludge. J Microbiol 48:275–283

    Article  CAS  Google Scholar 

  28. Zhang Q, Yang W, Ngo H, Guo W, Jin P, Dzakpasu M, Yang SJ, Wang Q, Wang X, Ao D (2016) Current status of urban wastewater treatment plants in China. Environ Int 92–93:11–22

    Article  Google Scholar 

  29. Zhao D, Huang R, Zeng J, Yu Z, Liu P, Cheng S, Wu QL (2014) Pyrosequencing analysis of bacterial community and assembly in activated sludge samples from different geographic regions in China. Appl Microbiol Biotechnol 98:9119–9128

    Article  CAS  Google Scholar 

  30. Zhou Z, Qiao W, Xing C, An Y, Shen X, Ren W, Jiang L, Wang L (2015) Microbial community structure of anoxic-oxic-settling-anaerobic sludge reduction process revealed by 454-pyrosequencing. Chem Eng J 266:249–257

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation of China (3176110) and Natural Science Foundation of Gansu Province (148RJZA046).

Author information

Authors and Affiliations

  1. School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, China

    Xiao-Hu Kang, Yan Leng & Xiao-Ying Zeng

  2. School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, China

    Mafiana Macdonald O & Shi-Weng Li

  3. Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province, Northwest Institute of Eco-Environment and Resource, Chinese Academy of Sciences, Lanzhou, 730070, China

    Shi-Weng Li

Authors
  1. Xiao-Hu Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yan Leng
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mafiana Macdonald O
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiao-Ying Zeng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shi-Weng Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shi-Weng Li.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 13 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kang, XH., Leng, Y., O, M.M. et al. The seasonal changes of core bacterial community decide sewage purification in sub-plateau municipal sewage treatment plants. Bioprocess Biosyst Eng 43, 1609–1617 (2020). https://doi.org/10.1007/s00449-020-02352-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-020-02352-2

Keywords

Search

Navigation