Abstract
Transfer RNAs (tRNAs) are heavily decorated with post-transcriptional modifications during their biosynthesis. To fulfil their functions within cells, tRNAs undergo a tightly controlled biogenesis process leading to the formation of mature tRNAs. In particular, the introduction of post-transcriptional modifications in tRNAs is controlled and influenced by multiple factors. In turn, tRNA biological functions are often modulated by their modifications. Although modifications play essential roles in tRNA biology, methods to directly detect their introduction during tRNA maturation are rare and do not easily provide information on the temporality of modification events. To obtain information on the tRNA maturation process, we have developed a methodology, using NMR as a tool to monitor tRNA maturation in a non-disruptive and continuous fashion in cellular extracts. Here we report the 1H,15N chemical shift assignments of imino groups in three forms of the yeast tRNAPhe differing in their modification content. These assignments are a prerequisite for the time-resolved NMR monitoring of yeast tRNAPhe maturation in yeast extracts.
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Acknowledgements
The authors are grateful to Christel Le Bon for ensuring the best performance of the NMR infrastructure at the IBPC. The authors acknowledge access to the biomolecular NMR platform of the IBPC that is supported by the CNRS, the Labex DYNAMO (ANR-11-LABX-0011), the Equipex CACSICE (ANR-11-EQPX-0008) and the Conseil Régional d’Île-de-France (SESAME Grant). They also acknowledge access to the NMR infrastructure of the Paris Descartes University that is supported by grants from the Conseil Régional d’Île-de-France (SESAME Grant), the European Union (FEDER) and the Paris Descartes University. This work was supported by the ANR NMR-VitAmin (ANR-14-CE09-0012).
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Catala, M., Gato, A., Tisné, C. et al. 1H, 15N chemical shift assignments of the imino groups of yeast tRNAPhe: influence of the post-transcriptional modifications. Biomol NMR Assign 14, 169–174 (2020). https://doi.org/10.1007/s12104-020-09939-6
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DOI: https://doi.org/10.1007/s12104-020-09939-6