Abstract
Pasteurella multocida is the main cause of haemorrhagic septicaemia (HS) outbreak in livestock, such as cattle and buffaloes. Conventional vaccines such as alum-precipitated or oil-adjuvant broth bacterins were injected subcutaneously to provide protection against HS. However, the immunity developed is only for short term and needed to be administered frequently. In our previous study, a short gene fragment from Pasteurella multocida serotype B was obtained via shotgun cloning technique and later was cloned into bacterial expression system. pQE32-ABA392 was found to possess immunogenic activity towards HS when tested in vivo in rat model. In this study, the targeted gene fragment of ABA392 was sub-cloned into a DNA expression vector pVAX1 and named as pVAX1-ABA392. The new recombinant vaccine was stable and expressed on mammalian cell lines. Serum sample collected from a group of vaccinated rats for ELISA test shows that the antibody in immunized rats was present at high titer and can be tested as a vaccine candidate with challenge in further studies. This successful recombinant vaccine is immunogenic and potentially could be used as vaccine in future against HS.
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Acknowledgement
This research was supported by Postgraduate Research Grant (PPP) PG237-2014B Grant under University of Malaya. The authors are grateful to all Molecular Bacteriology Toxicology members for their laboratory assistance during this study.
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SI, SC, RDV, KTL and CWKS conceived and planned the research. SC and KTL performed the research. SC, NK and SI contributed to the analyzed of the result. HAR and NNR provided critical feedback and contributed new methods. SC and SI took the lead in writing manuscript. All authors provided critical feedback and helped shape the research, analysis and manuscript.
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Chelliah, S., Velappan, R.D., Lim, K.T. et al. Potential DNA Vaccine for Haemorrhagic Septiceamia Disease. Mol Biotechnol 62, 289–296 (2020). https://doi.org/10.1007/s12033-020-00244-0
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DOI: https://doi.org/10.1007/s12033-020-00244-0