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De novo sequence of the mitochondrial genome of Tyrophagus putrescentiae (Acari: Sarcoptiformes) including 22 tRNA sequences and the largest non-coding region

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Abstract

In this study, we de novo sequenced and analyzed the circular mitochondrial genome (mitogenome) of Tyrophagus putrescentiae. It was 14,156 bp long and contained a complete set of 37 genes, contrary to the initial published sequences; it included 22 tRNA sequences and the largest non-coding region. The mtDNA gene order of T. putrescentiae was found to be identical to that of Aleuroglyphus ovatus, Caloglyphus berlesei, and Rhizoglyphus robini (all Acaroidea). Most tRNAs of T. putrescentiae lack at least a D-arm or T-arm. Tyrophagus putrescentiae tRNAs also shared considerable structural and sequence similarity with the tRNAs of other reported Acaroidea species that have the full set of tRNAs. The largest non-coding region was located between trnF and trnS1, and it contained a microsatellite-like (AT)n sequence, short palindromic sequences, and several hairpin loops, as observed in other reported Acaroidea species (excepting Tyrophagus longior).

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Acknowledgements

We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript and the authors would like to acknowledge the support by the Natural science Foundation of China (No. 31870352) and the Youth Talent Support of Wannan Medical College (No. wyqnyx201902).

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Authors and Affiliations

  1. Department of Health Inspection and Quarantine, Wannan Medical College, Wuhu, 241002, China

    Wei-Xi Fang, En-Tao Sun, Dong-Dong Tao, Yan Wang, Jiao-Yang Xu, Yu Fang & Chang-Jiang Ye

  2. Department of Pathology, Wannan Medical College, Wuhu, China

    Fang-Yuan Dong & Xue-Bing Zhan

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  1. Wei-Xi Fang
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Correspondence to En-Tao Sun.

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Mitochondrial genome organization of Tyrophagus putrescentiae. Additional file1 (DOCX 21 kb)

10493_2020_477_MOESM2_ESM.docx

Alignment of the inferred secondary structures of tRNA genes from Tyrophagus putrescentiae (Tp) and three other Acaroidea mites: Aleuroglyphus ovatus (Ao), Caloglyphus berlesei (Cb), Rhizoglyphus robini (Rr). Conserved nucleotides are shaded in gray across these four Acaroidea mites. Nucleotides that pair at the arms (acceptor arm, D arm, anticodon arm, and T arm) are underlined. Sequences of anticodons are in boldface. Alignment of the inferred secondary structures of tRNA genes from T. putrescentiae (Tp) and three other Acaroidea mites: A. ovatus (Ao), C. berlesei (Cb), R. robini (Rr). Additional file2 (DOCX 58 kb)

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Fang, WX., Dong, FY., Sun, ET. et al. De novo sequence of the mitochondrial genome of Tyrophagus putrescentiae (Acari: Sarcoptiformes) including 22 tRNA sequences and the largest non-coding region. Exp Appl Acarol 80, 521–530 (2020). https://doi.org/10.1007/s10493-020-00477-3

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