Abstract
Main conclusion
CYP722C from cotton, a homolog of the enzyme involved in orobanchol synthesis in cowpea and tomato, catalyzes the conversion of carlactonoic acid to 5-deoxystrigol.
Abstract
Strigolactones (SLs) are important phytohormones with roles in the regulation of plant growth and development. These compounds also function as signaling molecules in the rhizosphere by interacting with beneficial arbuscular mycorrhizal fungi and harmful root parasitic plants. Canonical SLs, such as 5-deoxystrigol (5DS), consist of a tricyclic lactone ring (ABC-ring) connected to a methylbutenolide (D-ring). Although it is known that 5DS biosynthesis begins with carlactonoic acid (CLA) derived from β-carotene, the enzyme that catalyzes the conversion of CLA remains elusive. Recently, we identified cytochrome P450 (CYP) CYP722C as the enzyme that catalyzes direct conversion of CLA to orobanchol in cowpea and tomato (Wakabayashi et al., Sci Adv 5:eaax9067, 2019). Orobanchol has a different C-ring configuration from that of 5DS. The present study aimed to characterize the homologous gene, designated GaCYP722C, from cotton (Gossypium arboreum) to examine whether this gene is involved in 5DS biosynthesis. Expression of GaCYP722C was upregulated under phosphate starvation, which is an SL-producing condition. Recombinant GaCYP722C was expressed in a baculovirus-insect cell expression system and was found to catalyze the conversion of CLA to 5DS but not to 4-deoxyorobanchol. These results strongly suggest that GaCYP722C from cotton is a 5DS synthase and that CYP722C is the crucial CYP subfamily involved in the generation of canonical SLs, irrespective of the different C-ring configurations.
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Abbreviations
- CCD7:
-
CAROTENOID CLEAVAGE DIOXYGENASE 7
- CCD8:
-
CAROTENOID CLEAVAGE DIOXYGENASE 8
- CLA:
-
Carlactonoic acid
- D27:
-
DWARF 27
- 4DO:
-
4-Deoxyorobanchol
- 5DS:
-
5-Deoxystrigol
- MAX1:
-
MORE AXIALLY GROWTH 1
- SLs:
-
Strigolactones
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Acknowledgements
This work was supported, in part, by JST/JICA SATREPS (JPMJSA1607 to YS) and JSPS KAKENHI (25292065 and 19H02897 to YS).
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Supplementary Fig. S1
Sequence comparison of the transcript of the cloned GaCYP722C with Ga12G2447.1 from the Cotton Functional Genomics database. Supplementary Fig. S2 Alignment of amino acid sequences of CYP722C from cotton, tomato, cowpea and L. japonicus. Sequences were aligned using CLUSTAL Omega program. (DOCX 1034 kb)
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Wakabayashi, T., Shida, K., Kitano, Y. et al. CYP722C from Gossypium arboreum catalyzes the conversion of carlactonoic acid to 5-deoxystrigol. Planta 251, 97 (2020). https://doi.org/10.1007/s00425-020-03390-6
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DOI: https://doi.org/10.1007/s00425-020-03390-6