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Increased Myocardial Retention of Mesenchymal Stem Cells Post-MI by Pre-Conditioning Exercise Training

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Abstract

Stem cell (SC) therapy is a promising approach to improve post-myocardial infarction (MI) cardiac remodeling, but the proinflammatory microenvironment may lead to SC loss and, therefore, may have a negative impact on therapy. It appears that exercise training (ET) improves myocardial microenvironment for SC transplantation. Therefore, we tested the effect of ET on post-infarction retention of adipose-derived SCs (ADSCs) and its combined effects on the inflammatory microenvironment. Fischer-344 female rats were randomized to one of the following groups: Sham; sedentary coronary occlusion who did not receive ADSCs (sMI); sedentary coronary occlusion who received ADSCs; exercise coronary occlusion who received ADSCs. Rats were trained nine weeks prior to MI, followed by ADSCs transplantation. The MI led to left ventricle (LV) dilation and dysfunction, myocardial hypertrophy and fibrosis, and increased proinflammatory profile compared to Sham rats. Conversely, ADSCs transplanted rats exhibited, better morphological and functional LV parameters; inhibition of myocardial hypertrophy and fibrosis; and attenuation of proinflammatory cytokines (interleukins 1β and 10, tumor necrosis factor α, and transforming growth factor β) in the myocardium compared to sMI rats. Interestingly, ET enhanced the effect of ADSCs on interleukin 10 expression. There was a correlation between cytokine expression and myocardial ADSCs retention. The. ET enhanced the beneficial effects of ADSCs in infarcted myocardium, which was associated with higher ADSCs retention. These findings highlight the importance of ET in myocardial retention of ADSCs and attenuation of cardiac remodeling post-infarction. Cytokine analysis suggests improvement in ET-linked myocardial microenvironment based on its anti-inflammatory action.

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Acknowledgements

Authors are grateful to Editage company for English assistance and proofreading the article. This work was supported by São Paulo Research Foundation – FAPESP [09/54225-8, 15/11028-9, 2018/06865-7) and Brazilian National Council for Scientific and Technological Development – CNPq [305527/2017-7]. Funding sources had no involvement in study design or collection, analysis and interpretation of data.

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  1. Laboratory of Physiology and Cardiac Pathophysiology, Department of Medicine, Federal University of São Paulo, SP, São Paulo, Brazil

    Stella Souza Vieira, Ednei Luiz Antonio, Brunno Lemes de Melo, Paulo José Ferreira Tucci¹ & Andrey Jorge Serra

  2. Cummings Tufts Veterinary Medical Center, Tufts University, North Graafton, MA, USA

    Luís Felipe Neves dos Santos

  3. Biophotonics Applied to Health Sciences Postgraduate Program, Nove de Julho University, SP, São Paulo, Brazil

    Eduardo Tadeu Santana, Regiane Feliciano, José Antônio Silva Jr & Andrey Jorge Serra

  4. Department of Radiology and Oncology, University of São Paulo Medical School, SP, São Paulo, Brazil

    Fabio Luiz Navarro Marques, Daniele de Paula Faria & Carlos Alberto Buchpiguel

  5. Department of Medicine, Cardiology Division, Federal University of São Paulo, Rua Pedro de Toledo 781, SP, 03650-040, São Paulo, Brazil

    Andrey Jorge Serra

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Souza Vieira, S., Antonio, E.L., de Melo, B.L. et al. Increased Myocardial Retention of Mesenchymal Stem Cells Post-MI by Pre-Conditioning Exercise Training. Stem Cell Rev and Rep 16, 730–741 (2020). https://doi.org/10.1007/s12015-020-09970-z

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