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A Study of Some Biochemical Properties of Ecto-ATPase (Nucleotidase) in Erythrocytes of the Black Sea Thornback Ray Raja clavata L.

  • Comparative and Ontogenic Biochemistry
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Abstract

Biochemical properties and some kinetic characteristics of whole-erythrocyte ecto-ATPase (EC 3.6.1.5) were studied in the Black Sea thornback ray Raja clavata L. It was shown that enzyme activity varied from 1.5 to 3.5 nmol Pi/min/μL of packed cells and peaked in the presence of 3.0-6.0 mM Mg2+. Ecto-ATPase exhibited broad substrate specificity towards splitting nucleoside triphosphates. The enzyme was tolerant to changes in ambient pH and exhibited maximum activity in the pH range from 5.5 to 7.7. The enzyme also showed high substrate affinity, with the Michaelis constant (KM) found to be 12.0 ± 1.0 μM and Vmax being 2.35 0.2 nmol Pi/ min/μL of packed cells. Erythrocyte ecto-ATPase in R. clavata was sustainable to erythrocyte storage at 4°C for four days and exhibited a tolerance to high concentrations of urea.

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Funding

This work was implemented within a state assignment no. АААА-А19-119012490045-0 “A study of fundamental physical, physiologo-biochemical, reproductive, population and behavioral characteristics of marine hydrobionts”.

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Authors and Affiliations

  1. T.I. Vyazemsky Karadag Scientific Station—Nature Reserve, Branch of the A.O. Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences, Feodosia, Kurortnoye, Russia

    Yu. A. Silkin, E. N. Silkina & M. Yu. Silkin

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  1. Yu. A. Silkin
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  2. E. N. Silkina
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  3. M. Yu. Silkin
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Correspondence to Yu. A. Silkin.

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All applicable international, national and institutional principles of handling and using experimental animals for scientific purposes were observed.

This study did not involve human subjects as research objects.

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Russian Text © The Author(s), 2020, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2020, Vol. 56, No. 1, pp. 32–41.

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Silkin, Y.A., Silkina, E.N. & Silkin, M.Y. A Study of Some Biochemical Properties of Ecto-ATPase (Nucleotidase) in Erythrocytes of the Black Sea Thornback Ray Raja clavata L.. J Evol Biochem Phys 56, 31–40 (2020). https://doi.org/10.1134/S0022093020010044

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