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Genetic diversity of Prorocentrum donghaiense population during bloom in the East China Sea revealed by microsatellite

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Abstract

Blooms of the dinoflagellate Prorocentrum donghaiense occur frequently along the East China coast and Yangtze River estuary and pose a potential threat to coastal ecosystems. However, information on the genetic diversity of P. donghaiense populations in the East China Sea remains limited despite its ecological significance. Here we developed nine microsatellite markers based on transcriptome sequencing and analyzed genetic diversity and structure of P. donghaiense population in the East China Sea. We found that the Nei’s genetic diversity index of P. donghaiense population varied from 0.019 to 0.551 with an average of 0.227, whereas Shannon’s index ranged from 0.055 to 0.968 with an average of 0.431. Moreover, four genetically distinct subpopulations were identified based on STRUCTURE analysis. These results clearly indicated that P. donghaiense population during the blooms had high genetic diversity in the East China Sea, which might form the basis for variability in phenotype of P. donghaiense population and empower its capability to adapt to changing environment. Collectively our findings uncovered the genetic distinctiveness in P. donghaiense populations between 2014 and 2016 and provide valuable insights into the genetic structure and diversity of P. donghaiense blooms.

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Acknowledgments

We thank Prof. Da-zhi Wang in Xiamen University, China, who kindly provides the transcriptome sequencing of P. donghaiense.

Funding

This work was supported by the National Key Research and Development Program of China (No. 2017YFC1404300) and the National Natural Science Foundation of China (Nos. 41230961, 41176088).

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  1. Hong-jia Huang and Chu-qiao Gan contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, College of Life Science and Technology, Jinan University, Guangzhou, 510632, China

    Hong-jia Huang, Chu-qiao Gan, Shao-wen Xiao, Cheng Zou, Srinivasan Balamurugan, Hong-ye Li, Jie-sheng Liu & Wei-dong Yang

  2. Department of Medical Technology, Medical College of Shaoguan University, Shaoguan, 512026, China

    Hong-jia Huang

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  1. Hong-jia Huang
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Huang, Hj., Gan, Cq., Xiao, Sw. et al. Genetic diversity of Prorocentrum donghaiense population during bloom in the East China Sea revealed by microsatellite. J Appl Phycol 32, 1851–1862 (2020). https://doi.org/10.1007/s10811-020-02113-4

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