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Anti-cancer effects of methanol-ethyl acetate partitioned fraction from Magnolia grandiflora in human non-small cell lung cancer H1975 cells

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Abstract

Non-small cell lung cancer (NSCLC) constitutes nearly 85% of all cases of lung cancer. Drug resistance, dose-limiting toxicity, and metastasis in NSCLC eventually reduce the efficacy of chemotherapeutics. In this study, we have shown that the methanol-ethyl acetate partitioned fraction from Magnolia grandiflora L. seeds (MEM) exhibit potential anti-cancer activities against NSCLC H1975 cells in vivo and in vitro. MEM significantly inhibited the proliferation of H1975 cells in a concentration- and time-dependent manner. Further, MEM exhibited potent anti-tumor efficacy and low toxicity in nude mice bearing H1975 tumors. Our study also showed that MEM could induce cellular apoptosis in H1975 cells by down-regulating the protein expression levels of Akt and p-Akt-473, and by increasing the ratio of Bax/Bcl-2. Also, MEM significantly inhibited metastasis-related cell invasion and migration of H1975 cells, which associated with the down-regulation of HIF-1α, MMP-2, and MMP-9 protein expression levels. Thus, our data shows that MEM may be an effective fraction of M. grandiflora in NSCLC treatment.

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Acknowledgements

This work was financially supported by the Education Department of Anhui Natural Science Research Project China under Grant (KJ2018A0232, KJ2019A0326); the Foundation of Bengbu Medical College (Byycx1911).

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  1. Hui Ma and Xiangjian Bai contributed equally to this work.

Authors and Affiliations

  1. School of Pharmacy, Bengbu Medical College, 233030, Bengbu, Anhui, China

    Hui Ma, Xiangjian Bai, Xiaolong Sun, Bohan Li, Meilin Zhu, Yiqun Dai, Qiang Huo, Hong-Mei Li & Cheng-Zhu Wu

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  1. Hui Ma
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Ma, H., Bai, X., Sun, X. et al. Anti-cancer effects of methanol-ethyl acetate partitioned fraction from Magnolia grandiflora in human non-small cell lung cancer H1975 cells. J Bioenerg Biomembr 52, 175–183 (2020). https://doi.org/10.1007/s10863-020-09828-6

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