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High prevalence of subclinical paratuberculosis in buffaloes (Bubalus bubalis) in Maranhão, Brazil

  • Veterinary Microbiology - Research Paper
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Abstract

Paratuberculosis is an infectious, chronic, and incurable disease that affects ruminants, causing enteritis and chronic granulomatous lymphadenitis, characterized by malabsorption syndrome, its agent is the Mycobacterium avium subsp. paratuberculosis (MAP). Thus, the objective of this work was to identify and characterize MAP in buffalo herds slaughtered in Baixada Maranhense region. Samples of intestines, mesenteric lymph nodes, and ileocecal valves were collected from 115 buffaloes slaughtered at Baixada Maranhense slaughterhouses to perform the diagnosis by histopathological examination using staining with Hematoxylin and Eosin (H&E) and Ziehl-Neelsen, bacterial isolation, and real-time PCR. In the histopathology by H&E staining, there was evidence suggestive of paratuberculosis in 30% (31/115) of the buffaloes. With Ziehl-Neelsen staining, acid-fast bacilli (AFB) were visualized in 27% (26/115) of the tissue samples analyzed. MAP was isolated in 4.3% (5/115) of the fecal samples subjected to bacterial culture. The samples inoculated in HEYM with mycobactin J produced colonies identified with MAP according to their own morphological characteristics such as round, white, smooth and slightly rough, alcohol-acid staining, and slow growth with 8 weeks of incubation and mycobactin dependence. The agent confirmation was performed in five bacterial isolates (4.3%) and 15 (13%) fragments of jejunum, ileum, and mesenteric lymph node by the IS900 real-time PCR technique. The results of the present study demonstrate the subclinical occurrence of paratuberculosis in flocks of buffalo slaughtered in slaughterhouses of Baixada Maranhense.

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Acknowledgments

The authors would like to thank the Fundação de Amparo à Pesquisa e ao Desenvolvimento Científico e Tecnológico do Maranhão (FAPEMA) by encouraging aimed at the IECT Project - 008/17.

Funding

The authors would like to thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação de Amparo à Pesquisa e ao Desenvolvimento Tecnológico do Maranhão (FAPEMA) for funding this research.

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Authors and Affiliations

  1. Clinics Department, State University of Maranhão, Cidade Universitária Paulo VI, Tirirical, P.O. BOX 09, São Luís, MA, 65055-970, Brazil

    Helder de Moraes Pereira

  2. Department of Pathology, State University of Maranhão, São Luís, MA, Brazil

    Hamilton Pereira Santos

  3. Post-graduate Program in Animal Science, State University of Maranhão, São Luís, MA, Brazil

    Emerson Antônio Araújo de Oliveira, Thais Bastos Rocha, Ítala Mayara Silva Araújo & Diego Moraes Soares

  4. Researcher the Program in Animal Sanitary Defense, State University of Maranhão, São Luís, MA, Brazil

    Felício Garino Junior

  5. Department of Veterinary Medicine, Rural Federal University of Pernambuco, Recife, PE, Brazil

    Pedro Paulo Feitosa de Albuquerque & Rinaldo Aparecido Mota

Authors
  1. Helder de Moraes Pereira
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  2. Hamilton Pereira Santos
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  3. Emerson Antônio Araújo de Oliveira
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  4. Thais Bastos Rocha
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  5. Ítala Mayara Silva Araújo
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  6. Diego Moraes Soares
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  7. Felício Garino Junior
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  8. Pedro Paulo Feitosa de Albuquerque
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  9. Rinaldo Aparecido Mota
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Corresponding author

Correspondence to Helder de Moraes Pereira.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethics approval

This study was approved by the Committee on Ethics and Animal Experimentation (CEEA/CVMP/UEMA), according to process no. 17/2014, approved on 24/11/2015.

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Responsible Editor: Miliane Moreira Soares de Souza.

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de Moraes Pereira, H., Santos, H.P., de Oliveira, E.A.A. et al. High prevalence of subclinical paratuberculosis in buffaloes (Bubalus bubalis) in Maranhão, Brazil. Braz J Microbiol 51, 1383–1390 (2020). https://doi.org/10.1007/s42770-020-00267-4

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  • DOI: https://doi.org/10.1007/s42770-020-00267-4

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