Abstract
The study deals with the last unexplored morphological group of the genus Hodophilus defined by absence of distinct odours, absence of yellow colours and absence of darker dots on the stipe. The phylogenetic reconstruction of the whole genus based on nrITS, nrLSU and RPB2 sequences placed all European members having these morphological characters in a monophyletic group defined here as a new section H. sect. Phaeophylli. The remaining European members of the genus are placed in two additional groups classified as section H. sect. Hodophilus and the new section H. sect. Micacei. Five species are recognised within section Phaeophylli which is typified by H. phaeophyllus that is lecto- and epitypified. Three new species belonging to this section are described: H. carpathicus, H. decurrentior and H. stramineus. Hodophilus decurrentior is the only species showing distinct morphological differences under the microscope. The identification of other species of the section depends mainly on the colour of basidiomata. An updated key to all European members of the genus is provided.
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References
Adamčík S, Looney BP, Birkebak JM, Jančovičová S, Adamčíková K, Marhold K, Matheny PB (2016) Circumscription of species of Hodophilus (Clavariaceae, Agaricales) in North America with naphthalene odour. Botany 94:941–956. https://doi.org/10.1139/cjb-2016-0091
Adamčík S, Jančovičová S, Looney BP, Adamčíková K, Birkebak JM, Moreau PA, Vizzini A, Matheny PB (2017a) Circumscription of species in the Hodophilus foetens complex (Clavariaceae, Agaricales) in Europe. Mycol Prog 16:47–62. https://doi.org/10.1007/s11557-016-1249-x
Adamčík S, Jančovičová S, Looney BP, Adamčíková K, Griffith GW, Læssøe T, Moreau PA, Vizzini A, Matheny PB (2017b) Hodophilus (Clavariaceae, Agaricales) species with dark dots on the stipe: more than one species in Europe. Mycol Prog 16:811–821. https://doi.org/10.1007/s11557-017-1318-9
Adamčík S, Dima B, Adamčíková K, Harries D, Læssøe T, Moreau P-A, Jančovičová S (2018) European Hodophilus (Clavariaceae, Agaricales) species with yellow stipe. Mycol Prog 17:1097–1111. https://doi.org/10.1007/s11557-018-1418-1
Arauzo S, Iglesias P (2018) Estudios en el género Hodophilus (Clavariaceae). Errotari 15:314–340
Arnolds E (1986) Notes on Hygrophoraceae IX. Camarophyllopsis Herink, an older name for Hygrotrama Sing. Mycotaxon 25:639–644
Arnolds E (1990) Camarophyllopsis. In: Bas C, Kuyper TW, Noordeloos ME, Vellinga EC (eds) Flora Agaricina Neerlandica, vol 2. A.A. Balkema, Rotterdam, Brookfield, pp 111–115
Birkebak JM, Mayor JR, Ryberg M, Matheny PB (2013) A systematic, morphological, and ecological overview of the Clavariaceae (Agaricales). Mycologia 105:896–911. https://doi.org/10.3852/12-070
Birkebak JM, Adamčík S, Matheny PB (2016) Multilocus phylogenetic reconstruction of the Clavariaceae (Agaricales) reveals polyphyly of the agaricoid members. Mycologia 108:860–868. https://doi.org/10.3852/15-370
Boertmann D (2012) Camarophyllopsis Herink. In: Knudsen H, Vesterholt J (eds) Funga Nordica, 2nd edn. Nordsvamp, Copenhagen, pp 242–244
Borchsenius F (2009) FastGap 1.2. Department of Bio-sciences, Aarhus University, Denmark. http://www.aubot.dk/FastGap_home.htm
Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Mol Ecol 2:113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
Gouy M, Guindon S, Gascuel O (2010) SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol Biol Evol 27:221–224. https://doi.org/10.1093/molbev/msp259
Hora FB, Orton PD (1955) Three new british agaric records. Trans Br Mycol Soc 38:400–404
Horak E (2005) Röhrlinge und Blätterpilze in Europa. Spektrum Akademischer Verlag, München
Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30:772–780. https://doi.org/10.1093/molbev/mst010
Katoh K, Misawa K, Kuma K, Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res 30:3059–3066. https://doi.org/10.1093/nar/gkf436
Katoh K, Kuma K, Toh H, Miyata T (2005) MAFFT version 5: improvement in accuracy of multiple sequence alignment. Nucleic Acids Res 33:511–518. https://doi.org/10.1093/nar/gki198
Kornerup A, Wanscher JH (1967) Methuen Handbook of Colour, 2nd edn. Methuen Co., London
Kovalenko AE, Malysheva EF, Morozova OV (2012) The genus Camarophyllopsis in Russia: new records and new species C. albofloccipes. Mikol Fitopatol 46:54–66 [In Russian]
Lange M, Hansen EB (1950) Notes on Danish Fungi. Friesia 4:61–65
Matheny PB (2005) Improving phylogenetic inference of mushrooms with RPB1 and RPB2 nucleotide sequences (Inocybe; Agaricales). Mol Phylogenetics Evol 35:1–20. https://doi.org/10.1016/j.ympev.2004.11.014
Moser M (1978) Keys to agarics and boleti. Roger Phillips, London
Nagy LG, Kocsubé S, Csanádi Z, Kovács GM, Petkovits T, Cs V, Papp T (2012) Re-mind the gap! Insertion – deletion data reveal neglected phylogenetic potential of the nuclear ribosomal internal transcribed spacer (ITS) of fungi. PLoS ONE 7:e49794. https://doi.org/10.1371/journal.pone.0049794
Printz P, Læssøe T (1986) Vokshatteslægten Camarophyllopsis i Danmark. Svampe 14:83–92
Romagnesi H (1971) Sur quelques espèces omphalioïdes d’agarics leucosporés. Bull trimest Soc mycol Fr 86:865–874
Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MRBAYES 3.2: Efficient Bayesian phylogenetic inference and model selection across a large model space. Syst Biol 61:539–542. https://doi.org/10.1093/sysbio/sys029
Silvestro D, Michalak I (2012) raxmlGUI: a graphical front-end for RAxML. Org Divers Evol 12:335–337. https://doi.org/10.1007/s13127-011-0056-0
Simmons MP, Ochoterena H, Carr TG (2001) Incorporation, relative homoplasy, and effect of gap characters in sequence-based phylogenetic analysis. Syst Biol 50:454–462. https://doi.org/10.1080/10635150120427
Singer R (1975) The Agaricales in modern taxonomy, 3rd edn. J. Cramer, Vaduz
Vellinga EC (1988) Glossary. In: Bas C, Kuyper TW, Noordeloos ME, Vellinga EC (eds) Flora Agaricina Neerlandica, vol 1. A.A. Balkema, Rotterdam, Brookfield, pp 54–69
White TJ, Bruns T, Taylor LS (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and application. Academic Press, Inc., San Diego, pp 322–315
Acknowledgements
The authors would like to thank Tanja Böhning, Tor Erik Brandrud, Rob Foster, David Harries, Andreas Gminder, Viktor Kučera, Jean-Marc Moingeon, Anna Molia and Erik Arnfred Thomsen for their contribution of specimens, photographs and/or assistance with field work. We also thank the staff and curators of the herbarium at K, LIP and O (including NorBOL staff) for loans of the herbarium material.
Funding
The research of SA, MC, KA and SJ was granted by the national grant Vega 02/0018/18 and a grant from Slovak-American Foundation to SA. The research of BD was partly supported by the ELTE Institutional Excellence Program (1783-3/2018/FEKUTSRAT) of the Hungarian Ministry of Human Capacities.
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Adamčík, S., Dima, B., Adamčíková, K. et al. Hodophilus phaeophyllus complex (Clavariaceae, Agaricales) is defined as new phylogenetic lineage in Europe. Mycol Progress 19, 111–125 (2020). https://doi.org/10.1007/s11557-019-01544-9
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DOI: https://doi.org/10.1007/s11557-019-01544-9