Abstract
Fungal co-culture is a strategy to induce the production of secondary metabolites by activating cryptic genes. We discovered the production of a new compound, talarodone A (1), along with five known compounds 2–6 in co-culture of Talaromyces pinophilus and Paraphaeosphaeria sp. isolated from soil collected in Miyazaki Prefecture, Japan. Among them, the productions of penicidones C (2) and D (3) were enhanced 27- and sixfold, respectively, by the co-culture. The structure of 3 should be represented as a γ-pyridol form with the reported chemical shifts, but not as a γ-pyridone form, based on DFT calculation.
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Acknowledgements
This work was supported by JSPS KAKENHI Grants 17H03994 (S.T.), 18K06719 (H.K.), and 18K14933 (Y. H.), from the Noda Institute for Scientific Research (S.T.), and Useful and Unique Natural Products for Drug Discovery and Development (UpRod), Program for Building Regional Innovation Ecosystems at Kumamoto University, Japan. We are grateful to Dr. K. Miyazaki and Dr. A. Kinoshita of Kyushu Research Center, Forestry and Forest Products Research Institute, Japan, for identification of the fungal isolates.
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Murakami, S., Hayashi, N., Inomata, T. et al. Induction of secondary metabolite production by fungal co-culture of Talaromyces pinophilus and Paraphaeosphaeria sp.. J Nat Med 74, 545–549 (2020). https://doi.org/10.1007/s11418-020-01400-1
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DOI: https://doi.org/10.1007/s11418-020-01400-1