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Integrin-α9 and Its Corresponding Ligands Play Regulatory Roles in Chronic Periodontitis

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Abstract

Integrin-α9 (ITGA9) and its corresponding ligands are involved in inflammatory and immune responses. The present study aimed to investigate whether ITGA9 participates in the development of chronic periodontitis (ChP) and to explore the underlying mechanisms. We collected gingival tissue and gingival crevicular fluid in vivo from patients to determine the levels of ITGA9 and its ligands. We cultured primary periodontal ligament cells (PDLCs) in vitro and applied small interfering RNA to knock down ITGA9 in order to analyze the changes of inflammatory cytokines and explore the related cellular signaling pathways. The expression level of ITGA9 was significantly higher in the gingiva of patients with ChP than that of healthy individuals. ITGA9 knockdown in the PDLCs inhibited the secretion of interleukin (IL)-1β, IL-6, and IL-8. Western blot analysis indicated that this change could be attributed to the regulation of the mitogen-activated protein kinase (MAPK) signaling pathway. ITGA9 plays a regulatory role in the homeostasis of ChP. The results of the present study provide potential insights into the treatment of periodontitis.

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Funding

The current work was supported by the National Natural Science Foundation of China (No. 81870776 and No. 81570946).

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Authors and Affiliations

  1. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China

    Shihan Xu, Chenxi Jiang, Huan Liu, Huihui Zhang, Haiqing Liao, Xiaoxuan Wang, Siqi Yao, Li Ma, Yi Guo & Zhengguo Cao

  2. Department of Periodontology, School and Hospital of Stomatology, Wuhan University, Wuhan, China

    Huan Liu, Huihui Zhang & Zhengguo Cao

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  1. Shihan Xu
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  2. Chenxi Jiang
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Correspondence to Zhengguo Cao.

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Xu, S., Jiang, C., Liu, H. et al. Integrin-α9 and Its Corresponding Ligands Play Regulatory Roles in Chronic Periodontitis. Inflammation 43, 1488–1497 (2020). https://doi.org/10.1007/s10753-020-01226-9

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