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Linking sensitivity of photosystem II to UV-B with chloroplast ultrastructure and UV-B absorbing pigments contents in A. thaliana L. phyAphyB double mutants

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Abstract

Our recent studies showed that A. thaliana L. phyAphyB double mutants (DM) grown under red light (RL) and white light (WL) had higher photosystem II (PSII) vulnerability to UV-B than wild type (WT). The present work was aimed at revealing the mechanistic basis for this difference by analyzing the content of UV-absorbing pigments (UAPs) and chloroplast ultrastructure in leaves of DM and WT grown under different light conditions, of different photoperiods (12, 16 and 24 h) and light quality (WL vs RL). The content of UAPs in leaves of WT plants grown under RL showed a strong dependence of photoperiod and decreased in a sequence 24 h > 16 h > 12 h. In all treatments, contents of UAPs were higher in WT compared to mutant plants. While 1 h of UV treatment had only a small impact on chloroplast ultrastructure, it substantially inhibited PSII activity (maximum and effective PSII quantum yields). In all treatments, the UV-induced decreases of PSII activities were compared with each other. At any photoperiod, decreases in PSII activity were smaller in WT compared to that in mutant plants. Higher UAPs contents led to lesser PSII inhibition, while low UAPs contents resulted in strong decline in PSII activity. The results demonstrate that content of UAPs significantly contributes to PSII resistance to short-time UV-B exposures, and decreased content of UAPs in phytochrome double mutant can explain the reduced PSII resistance of these plants to UV-B radiation.

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Abbreviations

Car:

Carotenoids

Chl:

Chlorophyll

DM:

Phytochrome A, phytochrome B double mutant

PA:

Photosynthetic apparatus

PSII:

Photosystem II

PhyB:

Phytochrome B

PhyA:

Phytochrome A

RL:

Red light

UAPs:

UV-absorbing pigments

WL:

White light

WTv:

Wild type

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Acknowledgements

The work was supported by Grants from the Russian Foundation for Basic Research (Nos: 18-34-00613; 17-04-01289)

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Author notes

  1. Vladimir D. Kreslavski and Xin Huang are equal first author.

Authors and Affiliations

  1. Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino, Moscow Region, Russia, 142290

    Vladimir D. Kreslavski, Alexandra Khudyakova, Galina Shirshikova, Sergey K. Zharmukhamedov & Suleyman I. Allakhverdiev

  2. Controlled Photobiosynthesis Laboratory, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, Russia, 127276

    Vladimir D. Kreslavski & Suleyman I. Allakhverdiev

  3. International Research Centre for Environmental Membrane Biology, Foshan University, Foshan, 528000, China

    Xin Huang, Xuewen Li, Chenrong Nie & Sergey Shabala

  4. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya Street 3, Pushchino, Moscow Region, Russia, 142290

    Galina Semenova

  5. Research Institute of Crop Husbandry, Ministry of Agriculture, Pirshagi Settl., Sovkhoz 2, 1098, Baku, Azerbaijan

    Nizami Hummatov

  6. College of Science, King Saud University, Riyadh, Saudi Arabia

    Suleyman I. Allakhverdiev

  7. Department of Molecular and Cell Biology, Moscow Institute of Physics and Technology, Institutsky lane 9, Dolgoprudny, Moscow Region, Russia, 141700

    Suleyman I. Allakhverdiev

  8. Bionanotechnology Laboratory, Institute of Molecular Biology and Biotechnology, National Academy of Sciences, Matbuat Avenue 2a, 1073, Baku, Azerbaijan

    Suleyman I. Allakhverdiev

  9. Tasmanian Institute of Agriculture, University of Tasmania, 7001, Hobart, Tas, Australia

    Sergey Shabala

Authors
  1. Vladimir D. Kreslavski
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  2. Xin Huang
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Correspondence to Sergey Shabala.

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Kreslavski, V.D., Huang, X., Semenova, G. et al. Linking sensitivity of photosystem II to UV-B with chloroplast ultrastructure and UV-B absorbing pigments contents in A. thaliana L. phyAphyB double mutants. Plant Growth Regul 91, 13–21 (2020). https://doi.org/10.1007/s10725-020-00584-6

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