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Molecular characterization of clock-associated PSEUDO-RESPONSE REGULATOR 9 gene from Oncidium ‘Gower Ramsey’

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Abstract

The plant circadian clock regulates a number of central activities such as growth, flowering, abiotic and biotic stress responses, and metabolism. The PSEUDO-RESPONSE REGULATORs (PRRs) contribute to the regulation of the circadian oscillator and clock output processes. In order to elucidate the role of Oncidium PRR9, we isolated and characterized a PRR9 homolog (OnPRR9) from commercially important Oncidium ‘Gower Ramsey’. OnPRR9 encodes a 603-amino acid protein with a N-terminal REC domain and a C-terminal CCT domain. Phylogenic analysis displayed that the OnPRR9 was orthologous to other known or deduced PRR proteins at the sequence level. Examination of the transcriptional expression profile of the OnPRR9 revealed that it is subjected to circadian rhythms under the conditions of 12-h light and 12-h dark cycles (12:12LD) and continuous light (LL) but not the continuous dark (DD) conditions. Genetic complementation showed that it can partially rescue the flowering time defect of Arabidopsis mutant prr5/9 grown under the long-day (16L:8D) conditions. Overexpression analysis indicated that the OnPRR9 gene can promote flowering and inhibit hypocotyl elongation under the short-day (8L:16D) conditions. Expression pattern of the OnPRR9 gene in petals during flower development and senescence implied that it may play a role in the initiation of flower senescence. These results extend our understanding of the functional conservation of PRR9 and may provide a means of regulating flowering time of Oncidium.

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Acknowledgements

This work was funded by the Basic and Applied Basic Research Plans (Natural Science Field) for High-level Talents of Hainan Province in 2019 (2019RC147), the National Natural Science Foundation of China (No. 31560573), the Postgraduate Research and Innovation Projects in Hainan Province in 2019 (Hby2019-14, Hys2019140), and the Postgraduate Research and Innovation Projects of Crop Discipline in Hainan University (ZWCX2018030).

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  1. Cui-Ping Yang and Yu-Rong Tan have contributed equally to this work.

Authors and Affiliations

  1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan University, Haikou, 570228, Hainan Province, People’s Republic of China

    Cui-Ping Yang, Yu-Rong Tan, Bing-Yu Yan, Xiao-Xiao Gong, Dan Wang, Xuan Gao, Heng Zhang, Peng Wang, Shuang-Jiang Li, Yi Wang, Lu-Yao Zhou & Jin-Ping Liu

  2. Post-Entry Quarantine Station for Tropical Plant, Hainan Entry-Exit Inspection and Quarantine Bureau, Haikou, 570311, People’s Republic of China

    Ying-Wen Pan

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Contributions

CPY and YRT performed the experiment and wrote the manuscript. JPL designed the whole experiment. BYY, XXG, DW, XG, HZ, PW, SJL, YW and LYZ contributed in transgenic experiment. YWP helped collecting the plant samples.

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Correspondence to Jin-Ping Liu.

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Suppementary file Fig. S1. Nucleotide sequence and deduced amino acid sequence of the OnPRR9. (JPG 217 kb)

10725_2020_611_MOESM2_ESM.jpg

Suppementary file Fig. S2. The conserved domains of the OnPRR9 obtained by the NCBI's conserved domain database (CDD) analysis. (jpg 78 kb)

10725_2020_611_MOESM3_ESM.jpg

Suppementary file Fig. S3. The OnPRR9 complementation lines were confirmed by PCR analysis at DNA level (A) and RNA level (B). A. The expected fragments of OnPRR9 + GFP were detected in all four putative transgenic Arabidopsis lines (1-4). No amplified PCR product was detected in non-transgenic prr5/9 mutant (5) and blank control (6). B. The expected transcripts of Hygromycin were detected in all three putative transgenic Arabidopsis lines (1-3). No amplified PCR product was detected in non-transgenic prr5/9 mutant (4) and blank control (5). (jpg 36 kb)

10725_2020_611_MOESM4_ESM.jpg

Suppementary file Fig. S4. The OnPRR9 overexpression lines were confirmed by PCR analysis at DNA level (A) and RNA level (B). A. The expected fragments of OnPRR9 + GFP were detected in all three putative transgenic Arabidopsis lines (1-3). No amplified PCR product was detected in non-transgenic wild type Arabidopsis (4) and blank control (5). B. The expected transcripts of the OnPRR9 were detected in all three putative transgenic Arabidopsis lines (1-3). No amplified PCR product was detected in non-transgenic wild type Arabidopsis (4). (jpg 34 kb)

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Yang, CP., Tan, YR., Yan, BY. et al. Molecular characterization of clock-associated PSEUDO-RESPONSE REGULATOR 9 gene from Oncidium ‘Gower Ramsey’. Plant Growth Regul 91, 371–381 (2020). https://doi.org/10.1007/s10725-020-00611-6

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