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Phototrophic potential and form II ribulose-1,5-bisphosphate carboxylase/oxygenase expression in five organs of the fluted giant clam, Tridacna squamosa

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Abstract

Despite living in oligotrophic tropical waters, giant clams can grow to large sizes because they live in symbiosis with extracellular phototrophic dinoflagellates (zooxanthellae) and receive photosynthates from them. The physical presence of zooxanthellae in five organs (colorful outer mantle, whitish inner mantle, ctenidium, hepatopancreas and foot muscle) of Tridacna squamosa had been confirmed by microscopy, and high densities of zooxanthellae were detected in specific regions of the inner mantle and foot muscle. Symbiotic dinoflagellates use form II ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) to fix inorganic carbon during C3 photosynthesis. Using qPCR primers that were designed comprehensively against all known zooxanthellal form II RuBisCO gene sequences (rbcII) in existing databases, we demonstrated that the outer mantle of T. squamosa (TS) had the greatest phototrophic potential as reflected by its high zooxanthellal rbcII (TSZrbcII) transcript level, which varied among different regions of the outer mantle. The other four organs also expressed moderate levels of TSZrbcII, despite the lack of iridophores and direct light exposure. Importantly, light exposure led to significant increases in the protein abundance of TSZRBCII in the outer mantle but not the other four organs. Taken together, these results indicate that organs inside the mantle cavity had low phototrophic potentials, but zooxanthellae residing inside these organs might serve some unidentified functions to benefit the host.

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Acknowledgements

This study was supported by the Singapore Ministry of Education through Grants (R-154-000-A37-114 and R‐154‐000‐B69-114) to Ip YK.

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Authors and Affiliations

  1. Department of Biological Sciences, National University of Singapore, Kent Ridge, Singapore, 117543, Republic of Singapore

    Jeslyn S. T. Poo, Celine Y. L. Choo, Kum C. Hiong, Mel V. Boo, Wai P. Wong & Yuen K. Ip

  2. Natural Sciences and Science Education, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore, 637616, Republic of Singapore

    Shit F. Chew

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Institutional approval was not required for research on giant clams (National University of Singapore Institutional Animal Care and Use Committee). Nonetheless, all applicable international, national and/or institutional guidelines for the care and use of animals were followed.

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Poo, J.S.T., Choo, C.Y.L., Hiong, K.C. et al. Phototrophic potential and form II ribulose-1,5-bisphosphate carboxylase/oxygenase expression in five organs of the fluted giant clam, Tridacna squamosa. Coral Reefs 39, 361–374 (2020). https://doi.org/10.1007/s00338-020-01898-7

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