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Do fungal associates of co-occurring orchids promote seed germination of the widespread orchid species Gymnadenia conopsea?

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Abstract

Interactions with mycorrhizal fungi have been increasingly recognized as one of the most important ecological factors determining the distribution and local abundance of orchids. While some orchid species may interact with a variety of fungal associates, others are more specific in their choice of mycorrhizal partners. Moreover, orchids that co-occur at a given site, often associate with different partners, possibly to avoid competition and to allow stable coexistence. However, whether differences in mycorrhizal partners directly affect seed germination and subsequent protocorm formation remains largely unknown. In this research, we used in vitro germination experiments to investigate to what extent seed germination and protocorm formation of Gymnadenia conopsea was affected by the origin and identity of fungal associates. Fungi were isolated from G. conopsea and three other co-occurring orchid species (Dactylorhiza viridis (Coeloglossum viride), Herminium monorchis, and Platanthera chlorantha). In total, eight fungal associates, belonging to Tulasnellaceae, Ceratobasidiaceae, and Serendipitaceae, were successfully isolated and cultured. While all eight fungal strains were able to promote early germination of G. conopsea seeds, only fungal strain GS2, a member of the Ceratobasidiaceae isolated from G. conopsea itself, was able to promote protocorm formation and subsequent growth to a seedling. Two other fungal strains isolated from G. conopsea only supported seed germination until the protocorm formation stage. The other five fungal strains isolated from the co-occurring orchid species did not support seed germination beyond the protocorm stage. We conclude that, although G. conopsea is considered a mycorrhizal generalist that associates with a wide range of fungi during its adult life, it requires specific fungi to promote protocorm formation and growth to a seedling.

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Funding

This work was financially supported by CAMS Initiative for Innovative Medicine (2016-I2M-2-002).

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Authors and Affiliations

  1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China

    Yue Gao, Zeyu Zhao, Jiayao Li, Na Liu, Shunxing Guo & Xiaoke Xing

  2. KU Leuven, Department of Biology, Plant Conservation and Population Biology, B-3001, Leuven, Belgium

    Hans Jacquemyn

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  1. Yue Gao
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  2. Zeyu Zhao
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  3. Jiayao Li
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  4. Na Liu
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  5. Hans Jacquemyn
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  7. Xiaoke Xing
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Correspondence to Xiaoke Xing.

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Gao, Y., Zhao, Z., Li, J. et al. Do fungal associates of co-occurring orchids promote seed germination of the widespread orchid species Gymnadenia conopsea?. Mycorrhiza 30, 221–228 (2020). https://doi.org/10.1007/s00572-020-00943-1

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