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Prevalence of Helicobacter pylori babA, oipA, sabA, and homB genes in isolates from Chinese patients with different gastroduodenal diseases

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Abstract

Disease outcome is associated with virulence factors of Helicobacter pylori (H. pylori), which are partially attributed to the outer membrane protein (OMP). This study aimed to investigate the correlation between the four OMP genes (babA, oipA, sabA, and homB) and gastroduodenal diseases. One hundred and seventy-seven H. pylori strains were isolated from Chinese patients with different gastroduodenal diseases (49 chronic gastritis, 19 gastric ulcer, 33 gastric cancer, and 76 duodenal ulcer), 94 of which contained pathological information (41 superficial gastritis, 24 intestinal hyperplasia, and 29 gastric adenocarcinoma). The full-length amplification of babA, oipA, sabA, and homB genes was acquired and sequenced. Then, the genetic polymorphism was analyzed to compare with the reference strains from the GenBank database. Functional status and cluster analysis were also performed to evaluate the impact of genetic polymorphism on disease outcome. The prevalence of babA, oipA, sabA, and homB genes were 91.5%, 100%, 94.0%, and 95.5%, respectively. The four OMP genes were characterized by genetic polymorphism and in the status of positive selection (Ka/Ks> 1). The proportion of strains with functional status on for oipA and sabA gene was 100% and 76.2%, respectively. The sequences of four OMP genes were mainly clustered together with the East Asian references. The four OMP genes were not different in patients with gastroduodenal diseases and pathologic changes (P > 0.05). H. pylori babA, oipA, sabA, and homB genes were common in the Chinese populations, but did not seem to be involved in the development of gastroduodenal diseases.

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Funding

Funding was provided by the National Key Research and Development Program of China (No. 2016YFC1302201), National Natural Science Foundation of China (Nos. 81970502, 81860107, and 81260076), Leading Talent Training Plan of the Gan-Po Outstanding Talents 555 Project of Jiangxi Province (2010-3-61), Natural Science Foundation of Fujian Province of China (No. 2017J01347), and Special Funds for Graduate Student Innovation of Jiangxi Province (No. YC2017-B024).

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  1. Qiaoyun Zhao and Conghua Song are joint first authors of this paper.

Authors and Affiliations

  1. Department of Gastroenterology, First Affiliated Hospital of Nanchang University, No.17 Yongwaizheng Street, Donghu District, Nanchang, 330006, Jiangxi, China

    Qiaoyun Zhao, Conghua Song, Yang Yang & Yong Xie

  2. Microbiology Laboratory, Gastroenterology Institute of Jiangxi Province, Nanchang, 330006, Jiangxi, China

    Qiaoyun Zhao, Conghua Song, Yang Yang, Dongsheng Liu, Le Wang & Yong Xie

  3. Gastroenterology Research Center, Key Laboratory of Digestive Diseases of Jiangxi Province, Nanchang, 330006, Jiangxi, China

    Qiaoyun Zhao, Conghua Song, Yang Yang, Dongsheng Liu, Le Wang & Yong Xie

  4. Laboratory of Biochemistry and Molecular Biology, Jiangxi Institute of Medical Sciences, Nanchang, 330006, Jiangxi, China

    Ke Wang, Donghong Li & Nanjin Zhou

  5. Department of Gastroenterology, Affiliated Hospital of Putian University, Putian, 351100, Fujian, China

    Conghua Song

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  1. Qiaoyun Zhao
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Contributions

YX conceived and designed research. QZ and CS conducted the experiment and wrote the manuscript. KW, DL, YY, and NZ contributed new reagents and analytical tools. DL and LW contributed to the source of strains. All authors read and approved the manuscript.

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Correspondence to Yong Xie.

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Zhao, Q., Song, C., Wang, K. et al. Prevalence of Helicobacter pylori babA, oipA, sabA, and homB genes in isolates from Chinese patients with different gastroduodenal diseases. Med Microbiol Immunol 209, 565–577 (2020). https://doi.org/10.1007/s00430-020-00666-2

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