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Electroacupuncture Relieves Pain and Attenuates Inflammation Progression Through Inducing IL-10 Production in CFA-Induced Mice

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Abstract

The therapeutic effect of electroacupuncture (EA) on inflammatory pain has been well recognized clinically, but the mechanism is unclear. Interleukin-10 (IL-10), which is produced by regulatory T (Treg) cell, is a key anti-inflammatory cytokine for relieving inflammatory pain. Therefore, the aim of this study is to investigate whether EA could inhibit CFA-induced pain and attenuate inflammation progression by regulating the activation of immunocyte and inducing the expression of IL-10. In this study, mice were treated with EA (2/100 Hz, 2 mA) for five consecutive days after 1 day of CFA injection. The behavioral tests were measured and analyzed after the daily EA treatment; then, hind paw, spinal cord, and spleen tissues were prepared for assessment. The results showed that EA treatment significantly increased the mechanical threshold and thermal latency after CFA injection and boosted the expression of IL-10 in paw and spinal cord tissues. EA treatment promoted Treg cells; suppressed macrophage and neutrophils cells; reduced the expression of IL-1β, NLRP3, and TNF-α; and ultimately relieved inflammatory pain. The findings suggested that the analgesic and anti-inflammatory effect of EA treatment could be partially associated with suppression of pro-inflammatory cytokines mediated by induction of IL-10.

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Acknowledgments

We thank Prof. Ye Yang of Nanjing University of Chinese Medicine for his help with the operation on flow cytometry.

Funding

This work was supported by the National Natural Science Foundation of China (No. 81774210, 81574062), “Six Major Talent Summit” of Jiangsu Province (YY-033), Jiangsu Province “333 High-level Talents Cultivating Project” (2016), “Qing Lan Project” of Jiangsu Province and A Project Funded by the Nursing Priority Academic Program Development of Jiangsu Higher Education Institutions (2019YSHL046). The funders have no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Authors and Affiliations

  1. Nanjing University of Chinese Medicine, First Clinical Medical College, Nanjing, China

    Mei-ling Yu & Zhi-gang Lu

  2. Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, Nanjing, China

    Mei-ling Yu, Tao Zhang, Yu Cheng, Fen-fen Qin, Shu-ping Fu, Zhi-gang Lu & Sheng-feng Lu

  3. School of Medicine and Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, China

    Rui-de Wei

  4. Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China

    Jun-meng Wang

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  1. Mei-ling Yu
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Contributions

Conceived and designed the experiments: SFL ZGL MLY. Performed the experiments: MLY RDW SFL JMW TZ FFQ SPF. Analyzed the data: MLY SFL RDW. Wrote the paper: SFL MLY ZGL. All authors have read and agreed with the manuscript.

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Correspondence to Zhi-gang Lu or Sheng-feng Lu.

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All procedures were strictly carried out in accordance with the guidelines of the NIH Animal Care and Use Committee, and the study was approved by the Institutional Animal Care and Use Committee of Nanjing University of Chinese Medicine.

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Yu, Ml., Wei, Rd., Zhang, T. et al. Electroacupuncture Relieves Pain and Attenuates Inflammation Progression Through Inducing IL-10 Production in CFA-Induced Mice. Inflammation 43, 1233–1245 (2020). https://doi.org/10.1007/s10753-020-01203-2

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